| |  Online-Ressource |
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| Verfasst von: | Seling, Annekatrin [VerfasserIn]  |
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| | Siegel, Corinna [VerfasserIn] |
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| | Fingerle, Volker [VerfasserIn] |
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| | Jutras, Brandon L. [VerfasserIn] |
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| | Brissette, Catherine A. [VerfasserIn] |
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| | Skerka, Christine [VerfasserIn] |
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| | Wallich, Reinhard [VerfasserIn] |
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| | Zipfel, Peter F. [VerfasserIn] |
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| | Stevenson, Brian [VerfasserIn] |
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| | Kraiczy, Peter [VerfasserIn] |
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| Titel: | Functional characterization of Borrelia spielmanii outer surface proteins that interact with distinct members of the human factor H protein family and with plasminogen |
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| Verf.angabe: | Annekatrin Seling, Corinna Siegel, Volker Fingerle, Brandon L. Jutras, Catherine A. Brissette, Christine Skerka, Reinhard Wallich, Peter F. Zipfel, Brian Stevenson, and Peter Kraiczy |
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| Jahr: | 2010 |
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| Jahr des Originals: | 2009 |
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| Umfang: | 10 S. |
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| Fussnoten: | Published ahead of print on 26 October 2009 ; Gesehen am 19.05.2021 |
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| Titel Quelle: | Enthalten in: Infection and immunity |
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| Ort Quelle: | Washington, DC : Soc., 1970 |
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| Jahr Quelle: | 2010 |
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| Band/Heft Quelle: | 78(2010), 1, Seite 39-48 |
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| ISSN Quelle: | 1098-5522 |
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| Abstract: | Acquisition of complement regulator factor H (CFH) and factor H-like protein 1 (CFHL1) from human serum enables Borrelia spielmanii, one of the etiological agents of Lyme disease, to evade complement-mediated killing by the human host. Up to three distinct complement regulator-acquiring surface proteins (CRASPs) may be expressed by serum-resistant B. spielmanii, each exhibiting an affinity for CFH and/or CFHL1. Here, we describe the functional characterization of the 15-kDa CRASPs of B. spielmanii, members of the polymorphic Erp (OspE/F-related) protein family, that bind two distinct host complement regulators, CFH and factor H-related protein 1 (CFHR1), but not CFHL1. CFH bound to the B. spielmanii CRASPs maintained cofactor activity for factor I-mediated C3b inactivation. Three naturally occurring alleles of this protein bound CFH and CFHR1 while a fourth natural allele could not. Comparative sequence analysis of these protein alleles identified a single amino acid, histidine-79, as playing a significant role in CFH/CFHR1 binding, with substitution by an arginine completely abrogating ligand binding. The mutation of His-79 to Arg did not inhibit binding of plasminogen, another known ligand of this group of borrelial outer-surface proteins. |
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| DOI: | doi:10.1128/IAI.00691-09 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://dx.doi.org/10.1128/IAI.00691-09 |
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| | Volltext: https://iai.asm.org/content/78/1/39 |
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| | DOI: https://doi.org/10.1128/IAI.00691-09 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Amino Acid Sequence |
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| | Bacterial Outer Membrane Proteins |
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| | Borrelia |
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| | Complement Activation |
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| | Complement Factor H |
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| | Culture Media |
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| | Gene Expression Regulation, Bacterial |
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| | Humans |
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| | Molecular Sequence Data |
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| | Plasminogen |
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| | Protein Binding |
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| | Serum |
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| K10plus-PPN: | 1758131349 |
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| Verknüpfungen: | → Zeitschrift |
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Functional characterization of Borrelia spielmanii outer surface proteins that interact with distinct members of the human factor H protein family and with plasminogen / Seling, Annekatrin [VerfasserIn]; 2010 (Online-Ressource)
