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Status: Bibliographieeintrag

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Verfasst von:Seling, Annekatrin [VerfasserIn]   i
 Siegel, Corinna [VerfasserIn]   i
 Fingerle, Volker [VerfasserIn]   i
 Jutras, Brandon L. [VerfasserIn]   i
 Brissette, Catherine A. [VerfasserIn]   i
 Skerka, Christine [VerfasserIn]   i
 Wallich, Reinhard [VerfasserIn]   i
 Zipfel, Peter F. [VerfasserIn]   i
 Stevenson, Brian [VerfasserIn]   i
 Kraiczy, Peter [VerfasserIn]   i
Titel:Functional characterization of Borrelia spielmanii outer surface proteins that interact with distinct members of the human factor H protein family and with plasminogen
Verf.angabe:Annekatrin Seling, Corinna Siegel, Volker Fingerle, Brandon L. Jutras, Catherine A. Brissette, Christine Skerka, Reinhard Wallich, Peter F. Zipfel, Brian Stevenson, and Peter Kraiczy
Jahr:2010
Jahr des Originals:2009
Umfang:10 S.
Fussnoten:Published ahead of print on 26 October 2009 ; Gesehen am 19.05.2021
Titel Quelle:Enthalten in: Infection and immunity
Ort Quelle:Washington, DC : Soc., 1970
Jahr Quelle:2010
Band/Heft Quelle:78(2010), 1, Seite 39-48
ISSN Quelle:1098-5522
Abstract:Acquisition of complement regulator factor H (CFH) and factor H-like protein 1 (CFHL1) from human serum enables Borrelia spielmanii, one of the etiological agents of Lyme disease, to evade complement-mediated killing by the human host. Up to three distinct complement regulator-acquiring surface proteins (CRASPs) may be expressed by serum-resistant B. spielmanii, each exhibiting an affinity for CFH and/or CFHL1. Here, we describe the functional characterization of the 15-kDa CRASPs of B. spielmanii, members of the polymorphic Erp (OspE/F-related) protein family, that bind two distinct host complement regulators, CFH and factor H-related protein 1 (CFHR1), but not CFHL1. CFH bound to the B. spielmanii CRASPs maintained cofactor activity for factor I-mediated C3b inactivation. Three naturally occurring alleles of this protein bound CFH and CFHR1 while a fourth natural allele could not. Comparative sequence analysis of these protein alleles identified a single amino acid, histidine-79, as playing a significant role in CFH/CFHR1 binding, with substitution by an arginine completely abrogating ligand binding. The mutation of His-79 to Arg did not inhibit binding of plasminogen, another known ligand of this group of borrelial outer-surface proteins.
DOI:doi:10.1128/IAI.00691-09
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://dx.doi.org/10.1128/IAI.00691-09
 Volltext: https://iai.asm.org/content/78/1/39
 DOI: https://doi.org/10.1128/IAI.00691-09
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Amino Acid Sequence
 Bacterial Outer Membrane Proteins
 Borrelia
 Complement Activation
 Complement Factor H
 Culture Media
 Gene Expression Regulation, Bacterial
 Humans
 Molecular Sequence Data
 Plasminogen
 Protein Binding
 Serum
K10plus-PPN:1758131349
Verknüpfungen:→ Zeitschrift

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