| Online-Ressource |
Verfasst von: | Seling, Annekatrin [VerfasserIn]  |
| Siegel, Corinna [VerfasserIn]  |
| Fingerle, Volker [VerfasserIn]  |
| Jutras, Brandon L. [VerfasserIn]  |
| Brissette, Catherine A. [VerfasserIn]  |
| Skerka, Christine [VerfasserIn]  |
| Wallich, Reinhard [VerfasserIn]  |
| Zipfel, Peter F. [VerfasserIn]  |
| Stevenson, Brian [VerfasserIn]  |
| Kraiczy, Peter [VerfasserIn]  |
Titel: | Functional characterization of Borrelia spielmanii outer surface proteins that interact with distinct members of the human factor H protein family and with plasminogen |
Verf.angabe: | Annekatrin Seling, Corinna Siegel, Volker Fingerle, Brandon L. Jutras, Catherine A. Brissette, Christine Skerka, Reinhard Wallich, Peter F. Zipfel, Brian Stevenson, and Peter Kraiczy |
Jahr: | 2010 |
Jahr des Originals: | 2009 |
Umfang: | 10 S. |
Fussnoten: | Published ahead of print on 26 October 2009 ; Gesehen am 19.05.2021 |
Titel Quelle: | Enthalten in: Infection and immunity |
Ort Quelle: | Washington, DC : Soc., 1970 |
Jahr Quelle: | 2010 |
Band/Heft Quelle: | 78(2010), 1, Seite 39-48 |
ISSN Quelle: | 1098-5522 |
Abstract: | Acquisition of complement regulator factor H (CFH) and factor H-like protein 1 (CFHL1) from human serum enables Borrelia spielmanii, one of the etiological agents of Lyme disease, to evade complement-mediated killing by the human host. Up to three distinct complement regulator-acquiring surface proteins (CRASPs) may be expressed by serum-resistant B. spielmanii, each exhibiting an affinity for CFH and/or CFHL1. Here, we describe the functional characterization of the 15-kDa CRASPs of B. spielmanii, members of the polymorphic Erp (OspE/F-related) protein family, that bind two distinct host complement regulators, CFH and factor H-related protein 1 (CFHR1), but not CFHL1. CFH bound to the B. spielmanii CRASPs maintained cofactor activity for factor I-mediated C3b inactivation. Three naturally occurring alleles of this protein bound CFH and CFHR1 while a fourth natural allele could not. Comparative sequence analysis of these protein alleles identified a single amino acid, histidine-79, as playing a significant role in CFH/CFHR1 binding, with substitution by an arginine completely abrogating ligand binding. The mutation of His-79 to Arg did not inhibit binding of plasminogen, another known ligand of this group of borrelial outer-surface proteins. |
DOI: | doi:10.1128/IAI.00691-09 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://dx.doi.org/10.1128/IAI.00691-09 |
| Volltext: https://iai.asm.org/content/78/1/39 |
| DOI: https://doi.org/10.1128/IAI.00691-09 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | Amino Acid Sequence |
| Bacterial Outer Membrane Proteins |
| Borrelia |
| Complement Activation |
| Complement Factor H |
| Culture Media |
| Gene Expression Regulation, Bacterial |
| Humans |
| Molecular Sequence Data |
| Plasminogen |
| Protein Binding |
| Serum |
K10plus-PPN: | 1758131349 |
Verknüpfungen: | → Zeitschrift |
Functional characterization of Borrelia spielmanii outer surface proteins that interact with distinct members of the human factor H protein family and with plasminogen / Seling, Annekatrin [VerfasserIn]; 2010 (Online-Ressource)