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Verfasst von:Liu, Yifei [VerfasserIn]   i
 Cao, Liji [VerfasserIn]   i
 Hillengaß, Jens [VerfasserIn]   i
 Delorme, Stefan [VerfasserIn]   i
 Schlewitz, Gudrun [VerfasserIn]   i
 Govindarajan, Parameswari [VerfasserIn]   i
 Schnettler, Reinhard [VerfasserIn]   i
 Heiß, Christian [VerfasserIn]   i
 Bäuerle, Tobias [VerfasserIn]   i
Titel:Quantitative assessment of microcirculation and diffusion in the bone marrow of osteoporotic rats using VCT, DCE-MRI, DW-MRI, and histology
Verf.angabe:Yifei Liu, Liji Cao, Jens Hillengass, Stefan Delorme, Gudrun Schlewitz, Parameswari Govindarajan, Reinhard Schnettler, Christian Heiß and Tobias Bäuerle
E-Jahr:2013
Jahr:March 1, 2013
Umfang:9 S.
Teil:volume:54
 year:2013
 number:2
 pages:205-213
 extent:9
Fussnoten:Gesehen am 19.05.2021
Titel Quelle:Enthalten in: Acta radiologica
Ort Quelle:London : Sage, 1921
Jahr Quelle:2013
Band/Heft Quelle:54(2013), 2, Seite 205-213
ISSN Quelle:1600-0455
Abstract:BackgroundEtiologic and pathophysiologic role of functional bone marrow processes is not fully understood especially in the case of osteoporosis.PurposeTo investigate the role of vascularization and diffusion in rat models of osteoporosis through a cross-correlation between non-invasive in-vivo imaging and invasive ex-vivo imaging of bone, bone marrow, and in particular of microcirculation.Material and MethodsOsteoporosis was induced in rats by combining ovariectomy (OVX) with calcium and Vitamin D3 deficiency, or with glucocorticoid (dexamethasone). For comparison, controls underwent a sham surgery. In in-vivo investigations, animals (n = 36) were examined by volumetric CT (VCT) and MRI at 1, 3, or 12 months post surgery. Using VCT, bone morphology was monitored and relative bone density r within pelvis was extracted. With DCE-MRI and DW-MRI, parameters A (amplitude), Kep (exchange rate constant), and ADC (apparent diffusion coefficient) were acquired for regions of lumbar vertebrae, pelvis, and femur. In ex-vivo investigations, selective histological sections of pelvis were either stained with hematoxylin and eosin (HE stain) for quantifying vessel size and density or immunostained for collagen IV and a-smooth muscle actin to assess vessel maturity (SMA/collagen IV ratio).ResultsAfter 12 months, decrease in DCE-MRI parameter Kep was found in all locations of osteoporotic rats (strongest in femur and lumbar vertebrae) while no significant differences were seen for parameter A and DW-MRI parameter ADC. Furthermore, vessel rarefication and maturation were observed on the histological level in animals with osteoporotic phenotype. In particular in the pelvis, the osteoporotic individuals (irrespective of the osteoporosis inducers applied) exhibited decreased Kep, significantly reduced vessel density, significantly increased vessel maturity, as well as statistically unaltered A, ADC, and vessel diameter.ConclusionChanges in microcirculation but not diffusion in the bone marrow of osteoporotic rats are detected by DCE-MRI and DW-MRI due to vessel rarefication and maturation.
DOI:doi:10.1258/ar.2012.120508
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1258/ar.2012.120508
 DOI: https://doi.org/10.1258/ar.2012.120508
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:CT - quantitative
 MR - diffusion/perfusion
 MR - functional imaging
 osteoporosis
 Skeletal - axial/appendicular
K10plus-PPN:1758182547
Verknüpfungen:→ Zeitschrift

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