| |  Online-Ressource |
|---|
| Verfasst von: | Burhenne, Jürgen [VerfasserIn]  |
|---|
| | Riedel, Klaus-Dieter [VerfasserIn] |
|---|
| | Martin-Facklam, Meret [VerfasserIn] |
|---|
| | Mikus, Gerd [VerfasserIn] |
|---|
| | Haefeli, Walter E. [VerfasserIn] |
|---|
| Titel: | Highly sensitive determination of saquinavir in biological samples using liquid chromatography-tandem mass spectrometry |
|---|
| Verf.angabe: | Jürgen Burhenne, Klaus-Dieter Riedel, Meret Martin-Facklam, Gerd Mikus, Walter E. Haefeli |
|---|
| Jahr: | 2003 |
|---|
| Umfang: | 10 S. |
|---|
| Teil: | volume:784 |
|---|
| | year:2003 |
|---|
| | number:2 |
|---|
| | pages:233-242 |
|---|
| | extent:10 |
|---|
| Fussnoten: | Online 28 October 2002 ; Gesehen am 25.05.2021 |
|---|
| Titel Quelle: | Enthalten in: Journal of chromatography / B |
|---|
| Ort Quelle: | New York, NY [u.a.] : Science Direct, 1977 |
|---|
| Jahr Quelle: | 2003 |
|---|
| Band/Heft Quelle: | 784(2003), 2, Seite 233-242 |
|---|
| ISSN Quelle: | 1873-376X |
|---|
| Abstract: | A selective and sensitive method for the determination of the HIV protease inhibitor saquinavir in human plasma, saliva, and urine using liquid-liquid extraction and LC-MS-MS has been developed, validated, and applied to samples of a healthy individual. After extraction with ethyl acetate, sample extracts were chromatographed isocratically within 5 min on Kromasil RP-18. The drug was detected with tandem mass spectrometry in the selected reaction monitoring mode using an electrospray ion source and 2H5-saquinavir as internal standard. The limit of quantification was 0.05 ng/mL. The accuracy of the method varied between −1 and +10% (SD within-batch) and the precision ranged from +4 to +10% (SD batch-to-batch). The method is linear at least within 0.05 and 87.6 ng/mL. After a regular oral dose (600 mg) saquinavir concentrations were detectable for 48 h in plasma and were well correlated with saliva concentrations (r2=0.9348, mean saliva/plasma ratio 1:15.1). The method is well suited for low saquinavir concentrations in different matrices. |
|---|
| DOI: | doi:10.1016/S1570-0232(02)00803-6 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/S1570-0232(02)00803-6 |
|---|
| | Volltext: https://www.sciencedirect.com/science/article/pii/S1570023202008036 |
|---|
| | DOI: https://doi.org/10.1016/S1570-0232(02)00803-6 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| Sach-SW: | Saquinavir |
|---|
| K10plus-PPN: | 1758445440 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
Highly sensitive determination of saquinavir in biological samples using liquid chromatography-tandem mass spectrometry / Burhenne, Jürgen [VerfasserIn]; 2003 (Online-Ressource)
