Rapid and reliable genotyping of the C181T polymorphism in the bradykinin B2 receptor Gene

• Verfasst von: Weiß, Johanna [VerfasserIn]
• Verfasst von: Hesse, Christiane [VerfasserIn]
• Verfasst von: Haefeli, Walter E. [VerfasserIn]
• Titel: Rapid and reliable genotyping of the C181T polymorphism in the bradykinin B2 receptor Gene
• Verf.angabe: Johanna Weiss, Christiane Hesse and Walter Emil Haefeli
• Jahr: 2003
• Umfang: 3 S.
• Teil: volume:41
• Teil: year:2003
• Teil: number:3
• Teil: pages:281-283
• Teil: extent:3
• Fussnoten: Gesehen am 25.05.2021
• Titel Quelle: Enthalten in: Clinical chemistry and laboratory medicine
• Ort Quelle: Berlin [u.a.] : De Gruyter, 1998
• Jahr Quelle: 2003
• Band/Heft Quelle: 41(2003), 3, Seite 281-283
• ISSN Quelle: 1437-4331
• DOI: doi:10.1515/CCLM.2003.044
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1758448768

Abstract

Bradykinin is not only a mediator of pain and inflammation but also a potent vasodilator and is likely to contribute to the cardioprotective effects of angiotensin-converting enzyme inhibitors. The B 2 receptor (B 2 -R) mediating most of the inflammatory and cardiovascular effects of bradykinin represents a candidate gene likely involved in the complex genetic causes of common chronic disorders such as hypertension. The C 181 T polymorphism of the B 2 -R gene influences the potency of bradykinin and there is evidence of a possible role of this polymorphism in the development of hypertension and end-stage renal disease. We developed and validated a new, rapid, and reliable method for the detection of the C 181 T polymorphism based on the hybridization probes format on the LightCycler™, which is superior to the conventional PCR-RFLP method commonly used to detect this polymorphism. It allows the solid evaluation of large patient populations with the best time.