| |  Online-Ressource |
|---|
| Verfasst von: | Inverso, Donato [VerfasserIn]  |
|---|
| | Shi, Jingjing [VerfasserIn] |
|---|
| | Lee, Ki Hong [VerfasserIn] |
|---|
| | Jakab, Moritz [VerfasserIn] |
|---|
| | Ben-Moshe, Shani [VerfasserIn] |
|---|
| | Kulkarni, Shubhada [VerfasserIn] |
|---|
| | Schneider, Martin [VerfasserIn] |
|---|
| | Wang, Guanxiong [VerfasserIn] |
|---|
| | Komeili, Marziyeh [VerfasserIn] |
|---|
| | Argos Vélez, Paula [VerfasserIn] |
|---|
| | Riedel, Maria [VerfasserIn] |
|---|
| | Spegg, Carleen [VerfasserIn] |
|---|
| | Ruppert, Thomas [VerfasserIn] |
|---|
| | Schaeffer-Reiss, Christine [VerfasserIn] |
|---|
| | Helm, Dominic [VerfasserIn] |
|---|
| | Singh, Indrabahadur [VerfasserIn] |
|---|
| | Boutros, Michael [VerfasserIn] |
|---|
| | Chintharlapalli, Sudhakar [VerfasserIn] |
|---|
| | Heikenwalder, Mathias [VerfasserIn] |
|---|
| | Itzkovitz, Shalev [VerfasserIn] |
|---|
| | Augustin, Hellmut [VerfasserIn] |
|---|
| Titel: | A spatial vascular transcriptomic, proteomic, and phosphoproteomic atlas unveils an angiocrine Tie-Wnt signaling axis in the liver |
|---|
| Verf.angabe: | Donato Inverso, Jingjing Shi, Ki Hong Lee, Moritz Jakab, Shani Ben-Moshe, Shubhada R. Kulkarni, Martin Schneider, Guanxiong Wang, Marziyeh Komeili, Paula Argos Vélez, Maria Riedel, Carleen Spegg, Thomas Ruppert, Christine Schaeffer-Reiss, Dominic Helm, Indrabahadur Singh, Michael Boutros, Sudhakar Chintharlapalli, Mathias Heikenwalder, Shalev Itzkovitz, and Hellmut G. Augustin |
|---|
| E-Jahr: | 2021 |
|---|
| Jahr: | 25 May 2021 |
|---|
| Umfang: | 27 S. |
|---|
| Fussnoten: | Das PDF enthält zusätzlich einen Anhang ; Gesehen am 28.05.2021 |
|---|
| Titel Quelle: | Enthalten in: Developmental cell |
|---|
| Ort Quelle: | Cambridge, Mass. : Cell Press, 2001 |
|---|
| Jahr Quelle: | 2021 |
|---|
| Band/Heft Quelle: | 56(2021), 11 vom: Juni, Seite 1677-1693.e10 |
|---|
| ISSN Quelle: | 1878-1551 |
|---|
| Abstract: | Single-cell transcriptomics (scRNA-seq) has revolutionized the understanding of the spatial architecture of tissue structure and function. Advancing the “transcript-centric” view of scRNA-seq analyses is presently restricted by the limited resolution of proteomics and genome-wide techniques to analyze post-translational modifications. Here, by combining spatial cell sorting with transcriptomics and quantitative proteomics/phosphoproteomics, we established the spatially resolved proteome landscape of the liver endothelium, yielding deep mechanistic insight into zonated vascular signaling mechanisms. Phosphorylation of receptor tyrosine kinases was detected preferentially in the central vein area, resulting in an atypical enrichment of tyrosine phosphorylation. Prototypic biological validation identified Tie receptor signaling as a selective and specific regulator of vascular Wnt activity orchestrating angiocrine signaling, thereby controlling hepatocyte function during liver regeneration. Taken together, the study has yielded fundamental insight into the spatial organization of liver endothelial cell signaling. Spatial sorting may be employed as a universally adaptable strategy for multiomic analyses of scRNA-seq-defined cellular (sub)-populations. |
|---|
| DOI: | doi:10.1016/j.devcel.2021.05.001 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.devcel.2021.05.001 |
|---|
| | Volltext: https://www.sciencedirect.com/science/article/pii/S1534580721004019 |
|---|
| | DOI: https://doi.org/10.1016/j.devcel.2021.05.001 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| Sach-SW: | angiocrine factors |
|---|
| | liver endothelial cell (L-EC) |
|---|
| | phosphoproteomics |
|---|
| | proteomics |
|---|
| | Tie1 |
|---|
| | Tie2 |
|---|
| | transcriptomics |
|---|
| | vascular zonation |
|---|
| | Wnt |
|---|
| K10plus-PPN: | 1759098337 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
¬A¬ spatial vascular transcriptomic, proteomic, and phosphoproteomic atlas unveils an angiocrine Tie-Wnt signaling axis in the liver / Inverso, Donato [VerfasserIn]; 25 May 2021 (Online-Ressource)
