| |  Online-Ressource |
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| Verfasst von: | Heinold, Andreas [VerfasserIn]  |
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| | Kuehl, Boris [VerfasserIn] |
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| | Brenner-Weiß, Gerald [VerfasserIn] |
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| | Opelz, Gerhard [VerfasserIn] |
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| | Tran, Thuong Hien [VerfasserIn] |
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| Titel: | Sequential analysis by immunoprecipitation-MALDI-TOF |
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| Titelzusatz: | a novel method for detection and identification of alloantibody specificities |
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| Verf.angabe: | Andreas Heinold, Boris Kuehl, Gerald Brenner-Weiss, Gerhard Opelz, Thuong Hien Tran |
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| E-Jahr: | 2010 |
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| Jahr: | 21 February 2010 |
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| Umfang: | 6 S. |
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| Teil: | volume:71 |
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| | year:2010 |
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| | number:5 |
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| | pages:462-467 |
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| | extent:6 |
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| Fussnoten: | Gesehen am 02.06.2021 |
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| Titel Quelle: | Enthalten in: Human immunology |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier Science, 1980 |
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| Jahr Quelle: | 2010 |
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| Band/Heft Quelle: | 71(2010), 5, Seite 462-467 |
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| ISSN Quelle: | 1879-1166 |
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| Abstract: | Alloantibodies are known to influence transplant outcomes. Apart from human leukocyte antigens (HLA), non-HLA targets have been suggested to play a significant role, but little is known about their nature. Here, we present a novel method for identification and characterization of cell surface antigens bound by alloreactive antibodies. Our method consists of 2 consecutive steps: first, immunoprecipitation of cell surface proteins is carried out with serum and, second, matrix-assisted laser desorption/ionization-time-of-flight is used to fingerprint the precipitated cell-surface proteins. As an example, we performed immunoprecipitation with peripheral blood lymphocytes, which had been incubated with an alloreactive serum; immune complexes were coupled to protein-G beads and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis; differential protein fractions were then analyzed by matrix-assisted laser desorption/ionization-time-of-flight. The method was validated with serum as well as with plasmapheresis material, which contained antibodies of known HLA specificities, demonstrating its applicability for clinical use. |
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| DOI: | doi:10.1016/j.humimm.2010.02.006 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.humimm.2010.02.006 |
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| | Volltext: https://www.sciencedirect.com/science/article/abs/pii/S019888591000042X?via%3Dihub |
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| | DOI: https://doi.org/10.1016/j.humimm.2010.02.006 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Amino Acid Sequence |
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| | Antibody Specificity |
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| | Electrophoresis, Polyacrylamide Gel |
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| | HLA Antigens |
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| | Humans |
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| | Immunoprecipitation |
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| | Isoantibodies |
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| | Isoantigens |
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| | Molecular Sequence Data |
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| | Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
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| K10plus-PPN: | 1759454095 |
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| Verknüpfungen: | → Zeitschrift |
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Sequential analysis by immunoprecipitation-MALDI-TOF / Heinold, Andreas [VerfasserIn]; 21 February 2010 (Online-Ressource)
