| |  Online-Ressource |
|---|
| Verfasst von: | Wallich, Reinhard [VerfasserIn]  |
|---|
| | Jahraus, Oliver [VerfasserIn] |
|---|
| | Stehle, Thomas [VerfasserIn] |
|---|
| | Tran, Thi Thanh Thao [VerfasserIn] |
|---|
| | Brenner, Christiane [VerfasserIn] |
|---|
| | Hofmann, Heidelore [VerfasserIn] |
|---|
| | Gern, Lise [VerfasserIn] |
|---|
| | Simon, Markus M. [VerfasserIn] |
|---|
| Titel: | Artificial-infection protocols allow immunodetection of novel Borrelia burgdorferi antigens suitable as vaccine candidates against Lyme disease |
|---|
| Verf.angabe: | Reinhard Wallich, Oliver Jahraus, Thomas Stehle, Thi Thanh Thao Tran, Christiane Brenner, Heidelore Hofmann, Lise Gern, and Markus M. Simon |
|---|
| Jahr: | 2003 |
|---|
| Umfang: | 12 S. |
|---|
| Teil: | volume:33 |
|---|
| | year:2003 |
|---|
| | number:3 |
|---|
| | pages:708-719 |
|---|
| | extent:12 |
|---|
| Fussnoten: | Gesehen am 02.06.2021 |
|---|
| Titel Quelle: | Enthalten in: European journal of immunology |
|---|
| Ort Quelle: | Weinheim : Wiley-VCH, 1971 |
|---|
| Jahr Quelle: | 2003 |
|---|
| Band/Heft Quelle: | 33(2003), 3, Seite 708-719 |
|---|
| ISSN Quelle: | 1521-4141 |
|---|
| Abstract: | Vaccination with recombinant outer surface protein A (OspA) from Borrelia burgdorferi provides excellent antibody-mediated protection against challenge with the pathogen in animal models and in humans. However, the bactericidal antibodies are ineffective in the reservoir host, since OspA is expressed by spirochetes only in the vector, but rarely, if at all, in mammals. Using an artificially generated immune serum (anti-10(8) spirochetes) with high protective potential for prophylactic and therapeutic treatment, we have now isolated from an expression library of B. burgdorferi (strain ZS7) three novel genes, zs7.a36, zs7.a66 and zs7.a68. All three genes are located, together with ospA/B, on the linear plasmid lp54, and are expressed in vitro and in ticks. At least temporarily two of them, ZS7.A36 and ZS7.A66, are also expressed during infection. The respective natural antigens are poorly immunogenic ininfected normal mice but elicited antibodies in Lyme disease patients. We show that recombinant preparations of ZS7.A36, ZS7.A66 and ZS7.A68 induce functional antibodies in rabbits capable of protecting immunodeficient mice against subsequent experimental infection. These findings suggest that all three recombinant antigens represent potential candidates for a "second generation" vaccine to prevent and/or cure Lyme disease. |
|---|
| DOI: | doi:10.1002/eji.200323620 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1002/eji.200323620 |
|---|
| | Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/eji.200323620 |
|---|
| | DOI: https://doi.org/10.1002/eji.200323620 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| Sach-SW: | Animals |
|---|
| | Antibodies, Bacterial |
|---|
| | Antigens, Bacterial |
|---|
| | Bacterial Vaccines |
|---|
| | Base Sequence |
|---|
| | Borrelia burgdorferi |
|---|
| | Female |
|---|
| | Humans |
|---|
| | Immune Sera |
|---|
| | Lyme Disease |
|---|
| | Mice |
|---|
| | Mice, SCID |
|---|
| | Molecular Sequence Data |
|---|
| | Rabbits |
|---|
| | Recombinant Proteins |
|---|
| | Ticks |
|---|
| | Vaccines, Synthetic |
|---|
| K10plus-PPN: | 175946967X |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
Artificial-infection protocols allow immunodetection of novel Borrelia burgdorferi antigens suitable as vaccine candidates against Lyme disease / Wallich, Reinhard [VerfasserIn]; 2003 (Online-Ressource)
