| |  Online-Ressource |
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| Verfasst von: | Herzberger, Pia [VerfasserIn]  |
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| | Siegel, Corinna [VerfasserIn] |
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| | Skerka, Christine [VerfasserIn] |
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| | Fingerle, Volker [VerfasserIn] |
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| | Schulte-Spechtel, Ulrike [VerfasserIn] |
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| | Wilske, Bettina [VerfasserIn] |
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| | Brade, Volker [VerfasserIn] |
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| | Zipfel, Peter F. [VerfasserIn] |
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| | Wallich, Reinhard [VerfasserIn] |
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| | Kraiczy, Peter [VerfasserIn] |
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| Titel: | Identification and characterization of the factor H and FHL-1 binding complement regulator-acquiring surface protein 1 of the Lyme disease spirochete Borrelia spielmanii sp. nov |
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| Verf.angabe: | Pia Herzberger, Corinna Siegel, Christine Skerka, Volker Fingerle, Ulrike Schulte-Spechtel, Bettina Wilske, Volker Brade, Peter F. Zipfel, Reinhard Wallich, Peter Kraiczy |
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| Jahr: | 2009 |
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| Umfang: | 14 S. |
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| Fussnoten: | Gesehen am 04.06.2021 |
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| Titel Quelle: | Enthalten in: International journal of medical microbiology |
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| Ort Quelle: | München : Elsevier, 2000 |
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| Jahr Quelle: | 2009 |
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| Band/Heft Quelle: | 299(2009), 2, Seite 141-154 |
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| ISSN Quelle: | 1618-0607 |
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| Abstract: | Borrelia spielmanii, one of the etiological agents of Lyme disease found in Europe, evades host complement-mediated killing by recruitment of the immune regulators factor H and FHL-1 from human serum. Serum-resistant and intermediate serum-resistant isolates express up to 3 distinct complement regulator-acquiring surface proteins (CRASPs) that bind factor H and/or FHL-1. The present study describes identification and functional characterization of BsCRASP-1 as the dominant factor H and FHL-1 binding protein of B. spielmanii. BsCRASP-1 is a 27.7kDa outer surface lipoprotein, which after processing has a predicted mass of 24.9kDa. BsCRASP-1 is encoded by a single copy gene, cspA, that maps to a linear plasmid of approximately 55kb. Ligand affinity blot techniques revealed that both native and recombinant BsCRASP-1 from different isolates can strongly bind FHL-1, but only weakly factor H. Deletion mutants of recombinant BsCRASP-1 were generated and a high-affinity binding site for factor H and FHL-1 was mapped to its carboxy-terminal 10-amino-acid residue domain. Similarly, the dominant binding site of factor H and FHL-1 was localized to short consensus repeats (SCRs) 5-7. Factor H and FHL-1 maintained cofactor activity for factor I-mediated C3b inactivation when bound to full-length BsCRASP-1 but not to a deletion mutant lacking the carboxy-terminal 10-amino-acid residue domain. In conclusion, BsCRASP-1 binds the host immune regulators factor H and FHL-1, and is suggested to represent a key molecule of B. spielmanii for complement resistance. Thus, BsCRASP-1 most likely contributes to persistence of B. spielmanii and to pathogenesis of Lyme disease. |
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| DOI: | doi:10.1016/j.ijmm.2008.06.005 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: http://dx.doi.org/10.1016/j.ijmm.2008.06.005 |
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| | Volltext: https://www.sciencedirect.com/science/article/abs/pii/S1438422108000817 |
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| | DOI: https://doi.org/10.1016/j.ijmm.2008.06.005 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Bacterial Outer Membrane Proteins |
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| | Bacterial Proteins |
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| | Binding Sites |
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| | Borrelia |
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| | Complement Factor H |
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| | DNA, Bacterial |
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| | Europe |
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| | Gene Deletion |
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| | Humans |
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| | Intracellular Signaling Peptides and Proteins |
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| | LIM Domain Proteins |
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| | Lyme Disease |
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| | Molecular Sequence Data |
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| | Molecular Weight |
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| | Muscle Proteins |
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| | Plasmids |
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| | Protein Binding |
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| | Protein Interaction Domains and Motifs |
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| | Protein Interaction Mapping |
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| | Sequence Analysis, DNA |
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| | Virulence Factors |
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| K10plus-PPN: | 1759783102 |
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| Verknüpfungen: | → Zeitschrift |
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Identification and characterization of the factor H and FHL-1 binding complement regulator-acquiring surface protein 1 of the Lyme disease spirochete Borrelia spielmanii sp. nov / Herzberger, Pia [VerfasserIn]; 2009 (Online-Ressource)
