| |  Online-Ressource |
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| Verfasst von: | Martin, Praxedis [VerfasserIn]  |
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| | Wallich, Reinhard [VerfasserIn] |
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| | Pardo, Julián [VerfasserIn] |
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| | Müllbacher, Arno [VerfasserIn] |
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| | Munder, Markus [VerfasserIn] |
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| | Modolell, Manuel [VerfasserIn] |
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| | Simon, Markus M. [VerfasserIn] |
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| Titel: | Quiescent and activated mouse granulocytes do not express granzyme A and B or perforin |
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| Titelzusatz: | similarities or differences with human polymorphonuclear leukocytes? |
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| Verf.angabe: | Praxedis Martin, Reinhard Wallich, Julian Pardo, Arno Müllbacher, Markus Munder, Manuel Modolell, and Markus M. Simon |
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| Jahr: | 2005 |
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| Umfang: | 8 S. |
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| Fussnoten: | Prepublished online as BloodFirst edition paper, July 5, 2005 ; Gesehen am 04.06.2021 |
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| Titel Quelle: | Enthalten in: Blood |
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| Ort Quelle: | Washington, DC : American Society of Hematology, 1946 |
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| Jahr Quelle: | 2005 |
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| Band/Heft Quelle: | 106(2005), 8, Seite 2871-2878 |
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| ISSN Quelle: | 1528-0020 |
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| Abstract: | Polymorphonuclear leukocytes have been shown to use a multitude of effector functions to combat pathogens and tumors, including enzymes, defensins, and toxic products such as oxygen radicals and nitrogen oxides. Recent studies provided evidence for the expression of granzymes (gzms) and perforin (perf) within the cytotoxic arsenal of human neutrophils, the validity of which was questioned by 2 subsequent studies. We have now used cytology, intracellular flow cytometry, enzymatic assays, immunoelectron microscopy, and quantitative reverse transcriptase-polymerase chain reaction to obtain evidence of the presence of gzms and/or perf in mouse Gr-1+ granulocyte populations. The data obtained clearly demonstrate that neither in vitro- nor in vivo-derived mouse granulocytes synthesize gzmA and gzmB or perf, even following infection/immunization with pathogens or pathogen-derived material. A parallel comparable analysis on the expression of gzmB in human neutrophils from 3 healthy control subjects and 4 patients with diverse diseases failed to detect gzmB expression. The data indicate that polymorphonuclear leukocytes from mice and humans lack the 3 cytotoxic effector molecules, gzmA, gzmB, and perf, generally associated with natural killer and cytotoxic T lymphocytes. |
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| DOI: | doi:10.1182/blood-2005-04-1522 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1182/blood-2005-04-1522 |
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| | DOI: https://doi.org/10.1182/blood-2005-04-1522 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Animals |
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| | Bone Marrow |
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| | Catalysis |
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| | Cell Differentiation |
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| | Cell Line |
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| | Gene Expression Regulation, Neoplastic |
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| | Granulocytes |
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| | Granzymes |
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| | Humans |
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| | Male |
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| | Membrane Glycoproteins |
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| | Mice |
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| | Mice, Knockout |
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| | Microscopy, Electron, Transmission |
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| | Neutrophils |
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| | Perforin |
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| | Phenotype |
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| | Pore Forming Cytotoxic Proteins |
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| | Serine Endopeptidases |
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| | Transcription, Genetic |
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| K10plus-PPN: | 175979564X |
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| Verknüpfungen: | → Zeitschrift |
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Quiescent and activated mouse granulocytes do not express granzyme A and B or perforin / Martin, Praxedis [VerfasserIn]; 2005 (Online-Ressource)
