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| Verfasst von: | Bozza, Matthias [VerfasserIn]  |
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| | De Roia, Alice [VerfasserIn] |
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| | Correia, Margareta [VerfasserIn] |
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| | Berger, Aileen [VerfasserIn] |
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| | Tuch, Alexandra [VerfasserIn] |
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| | Schmidt, Andreas [VerfasserIn] |
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| | Zörnig, Inka [VerfasserIn] |
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| | Jäger, Dirk [VerfasserIn] |
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| | Schmidt, Patrick [VerfasserIn] |
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| | Harbottle, Richard P. [VerfasserIn] |
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| Titel: | A nonviral, nonintegrating DNA nanovector platform for the safe, rapid, and persistent manufacture of recombinant T cells |
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| Verf.angabe: | Matthias Bozza, Alice De Roia, Margareta P. Correia, Aileen Berger, Alexandra Tuch, Andreas Schmidt, Inka Zörnig, Dirk Jager, Patrick Schmidt, Richard P. Harbottle |
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| E-Jahr: | 2021 |
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| Jahr: | 14 April 2021 |
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| Umfang: | 13 S. |
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| Begleitmaterial: | Suplementary material online abrufbar |
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| Fussnoten: | Gesehen am 04.06.2021 |
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| Titel Quelle: | Enthalten in: Science advances |
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| Ort Quelle: | Washington, DC [u.a.] : Assoc., 2015 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 7(2021), 16, Artikel-ID eabf1333, Seite 1-13 |
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| ISSN Quelle: | 2375-2548 |
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| Abstract: | The compelling need to provide adoptive cell therapy (ACT) to an increasing number of oncology patients within a meaningful therapeutic window makes the development of an efficient, fast, versatile, and safe genetic tool for creating recombinant T cells indispensable. In this study, we used nonintegrating minimally sized DNA vectors with an enhanced capability of generating genetically modified cells, and we demonstrate that they can be efficiently used to engineer human T lymphocytes. This vector platform contains no viral components and is capable of replicating extrachromosomally in the nucleus of dividing cells, providing persistent transgene expression in human T cells without affecting their behavior and molecular integrity. We use this technology to provide a manufacturing protocol to quickly generate chimeric antigen receptor (CAR)-T cells at clinical scale in a closed system and demonstrate their enhanced anti- tumor activity in vitro and in vivo in comparison to previously described integrating vectors. |
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| DOI: | doi:10.1126/sciadv.abf1333 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1126/sciadv.abf1333 |
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| | DOI: https://doi.org/10.1126/sciadv.abf1333 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1759798525 |
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| Verknüpfungen: | → Zeitschrift |
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¬A¬ nonviral, nonintegrating DNA nanovector platform for the safe, rapid, and persistent manufacture of recombinant T cells / Bozza, Matthias [VerfasserIn]; 14 April 2021 (Online-Ressource)
