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| Verfasst von: | Gavénis, Karsten [VerfasserIn]  |
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| | Schneider, Ulrich [VerfasserIn] |
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| | Wallich, Reinhard [VerfasserIn] |
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| | Mueller-Rath, Ralf [VerfasserIn] |
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| | Schmidt-Rohlfing, Bernhard [VerfasserIn] |
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| | Andereya, Stefan [VerfasserIn] |
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| Titel: | Effects of low concentrated BMP-7 administered by co-cultivation or plasmid transfection on human osteoarthritic chondrocytes |
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| Verf.angabe: | Karsten Gavenis, Ulrich Schneider, Reinhard Wallich, Ralf Mueller-Rath, Bernhard Schmidt-Rohlfing, Stefan Andereya |
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| Jahr: | 2010 |
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| Umfang: | 9 S. |
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| Teil: | volume:33 |
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| | year:2010 |
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| | number:6 |
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| | pages:339-347 |
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| | extent:9 |
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| Fussnoten: | Elektronische Reproduktion der Druck-Ausgabe ; First Published March 13, 2018 ; Gesehen am 04.06.2021 |
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| Titel Quelle: | Enthalten in: The international journal of artificial organs |
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| Ort Quelle: | Thousand Oaks, CA : Sage Publishing, 1978 |
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| Jahr Quelle: | 2010 |
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| Band/Heft Quelle: | 33(2010), 6, Seite 339-347 |
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| ISSN Quelle: | 1724-6040 |
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| Abstract: | INTRODUCTION: While BMP-7 has proven to be one of the most potent growth factors in cartilage tissue engineering, protein concentration and route of administration remain a matter of debate. Here we investigated the effects of a low concentration of BMP-7 on human osteoarthritic chondrocytes administered by protein co-cultivation and plasmid transfection. METHODS: Freshly released (P0) or in vitro propagated chondrocytes (P2) were cultivated in a collagen type-I gel for 3 weeks in vitro or in nude mice. Seeded chondrocytes were treated with 50 ng/mL BMP-7 directly added to the medium or were subject to transient BMP-7 plasmid transfection prior to gel cultivation. Untreated specimens served as a control. After recovery, samples were investigated by histological and immunohistochemical staining and real-time PCR. RESULTS: In vitro, collagen type-II protein production was enhanced, and it was stored mainly pericellularly. collagen type-II and aggrecan gene expression were enhanced in both treatment groups. After nude mouse cultivation, col-II protein production was further enhanced, but specimens of the BMP-7 transfection group revealed a clustering of col-II positive cells. Gene expression was strongly upregulated, chondrocyte number was increased and the differentiated phenotype prevailed. In general, freshly released chondrocytes (P0) proved to be superior to chondrocytes pre-amplified in vitro (P2). CONCLUSIONS: Both BMP-7 co-cultivation and plasmid transfection of human osteoarthritic chondrocytes led to improved cartilage repair tissue. Nevertheless, the col-II distribution following BMP-7 co-cultivation was homogeneous, while samples produced by transient transfection revealed a col-II clustering. |
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| DOI: | doi:10.1177/039139881003300602 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://dx.doi.org/10.1177/039139881003300602 |
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| | DOI: https://doi.org/10.1177/039139881003300602 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Animals |
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| | Bone Morphogenetic Protein 7 |
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| | Cell Culture Techniques |
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| | Chondrocytes |
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| | Coculture Techniques |
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| | Humans |
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| | Lipids |
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| | Mice |
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| | Mice, Inbred BALB C |
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| | Mice, Nude |
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| | Osteoarthritis |
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| | Plasmids |
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| | Transfection |
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| K10plus-PPN: | 1759813796 |
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| Verknüpfungen: | → Zeitschrift |
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Effects of low concentrated BMP-7 administered by co-cultivation or plasmid transfection on human osteoarthritic chondrocytes / Gavénis, Karsten [VerfasserIn]; 2010 (Online-Ressource)
