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Verfasst von:Großner, Tobias [VerfasserIn]   i
 Haberkorn, Uwe [VerfasserIn]   i
 Gotterbarm, Tobias [VerfasserIn]   i
Titel:18F- based quantification of the osteogenic potential of hMSCs
Verf.angabe:Tobias Grossner, Uwe Haberkorn and Tobias Gotterbarm
E-Jahr:2020
Jahr:17 October 2020
Umfang:13 S.
Teil:volume:21
 year:2020
 number:20
 elocationid:7692
 pages:1-13
 extent:13
Fussnoten:Im Titel ist die Zahl 18 hochgestellt ; Gesehen am 10.06.2021
Titel Quelle:Enthalten in: International journal of molecular sciences
Ort Quelle:Basel : Molecular Diversity Preservation International, 2000
Jahr Quelle:2020
Band/Heft Quelle:21(2020), 20, Artikel-ID 7692, Seite 1-13
ISSN Quelle:1422-0067
 1661-6596
Abstract:In bone tissue engineering, there is a constant need to design new methods for promoting in vitro osteogenic differentiation. Consequently, there is a strong demand for fast, effective and reliable methods to track and quantify osteogenesis in vitro. In this study, we used the radiopharmacon fluorine-18 (18F) to evaluate the amount of hydroxylapatite produced by mesenchymal stem cells (MSCs) in a monolayer cell culture in vitro. The hydroxylapatite bound tracer was evaluated using µ-positron emission tomography (µ-PET) scanning and activimeter analysis. It was therefore possible to determine the amount of synthesized mineral and thus to conclude the osteogenic potential of the cells. A Student’s t-test revealed a highly significant difference regarding tracer uptake between the osteogenic group and the corresponding control group (µ-PET p = 0.043; activimeter analysis p = 0.012). This tracer uptake showed a highly significant correlation with the gold standard of quantitative Alizarin Red staining (ARS) (r2 = 0.86) as well as with the absolute calcium content detected by inductively coupled plasma mass spectrometry (r2 = 0.81). The results showed that 18F labeling is a novel method to prove and quantify hydroxyapatite content in MSC monolayer cultures. The mineral layer remains intact for further analysis. This non-destructive in vitro method can be used to rapidly investigate bone tissue engineering strategies in terms of hydroxylapatite production, and could therefore accelerate the process of implementing new strategies in clinical practice.
DOI:doi:10.3390/ijms21207692
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.3390/ijms21207692
 Volltext: https://www.mdpi.com/1422-0067/21/20/7692
 DOI: https://doi.org/10.3390/ijms21207692
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:18F
 activimeter analysis
 mesenchymal stem cells
 osteogenic differentiation
 osteogenic quantification
 µ-PET
K10plus-PPN:1760206326
Verknüpfungen:→ Zeitschrift

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