Status: Bibliographieeintrag
Standort: ---
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| Online-Ressource |
Verfasst von: | Fiori, Agnese [VerfasserIn]  |
| Uhlig, Stefanie [VerfasserIn]  |
| Klüter, Harald [VerfasserIn]  |
| Bieback, Karen [VerfasserIn]  |
Titel: | Human adipose tissue-derived mesenchymal stromal cells inhibit CD4+ T cell proliferation and induce regulatory T cells as well as CD127 expression on CD4+CD25+ T cells |
Verf.angabe: | Agnese Fiori, Stefanie Uhlig, Harald Klüter and Karen Bieback |
E-Jahr: | 2021 |
Jahr: | 1 January 2021 |
Umfang: | 19 S. |
Fussnoten: | Gesehen am 10.06.2021 |
Titel Quelle: | Enthalten in: Cells |
Ort Quelle: | Basel : MDPI, 2012 |
Jahr Quelle: | 2021 |
Band/Heft Quelle: | 10(2021), 1, Artikel-ID 58, Seite 1-19 |
ISSN Quelle: | 2073-4409 |
Abstract: | Mesenchymal stromal cells (MSC) exert their immunomodulatory potential on several cell types of the immune system, affecting and influencing the immune response. MSC efficiently inhibit T cell proliferation, reduce the secretion of pro-inflammatory cytokines, limit the differentiation of pro-inflammatory Th subtypes and promote the induction of regulatory T cells (Treg). In this study, we analyzed the immunomodulatory potential of human adipose tissue-derived MSC (ASC), on CD4+ T cells, addressing potential cell-contact dependency in relation to T cell receptor stimulation of whole human peripheral blood mononuclear cells (PBMC). ASC were cultured with not stimulated or anti-CD3/CD28-stimulated PBMC in direct and transwell cocultures; PBMC alone were used as controls. After 7 days, cocultures were harvested and we analyzed: (1) the inhibitory potential of ASC on CD4+ cell proliferation and (2) phenotypic changes in CD4+ cells in respect of Treg marker (CD25, CD127 and FoxP3) expression. We confirmed the inhibitory potential of ASC on CD4+ cell proliferation, which occurs upon PBMC stimulation and is mediated by indoleamine 2,3-dioxygenase. Importantly, ASC reduce both pro- and anti-inflammatory cytokine secretion, without indications on specific Th differentiation. We found that stimulation induces CD25 expression on CD4+ cells and that, despite inhibiting overall CD4+ cell proliferation, ASC can specifically induce the proliferation of CD4+CD25+ cells. We observed that ASC induce Treg (CD4+CD25+CD127−FoxP3+) only in not stimulated cocultures and that ASC increase the ratio of CD4+CD25+CD127+FoxP3− cells at the expense of CD4+CD25+CD127−FoxP3− cells. Our study provides new insights on the interplay between ASC and CD4+ T cells, proposing that ASC-dependent induction of Treg depends on PBMC activation which affects the balance between the different subpopulations of CD4+CD25+ cells expressing CD127 and/or FoxP3. |
DOI: | doi:10.3390/cells10010058 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.3390/cells10010058 |
| Volltext: https://www.mdpi.com/2073-4409/10/1/58 |
| DOI: https://doi.org/10.3390/cells10010058 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | CD127 |
| CD4 T cells |
| immunomodulation |
| mesenchymal stromal cells |
| regulatory T cells |
K10plus-PPN: | 176021955X |
Verknüpfungen: | → Zeitschrift |
Human adipose tissue-derived mesenchymal stromal cells inhibit CD4+ T cell proliferation and induce regulatory T cells as well as CD127 expression on CD4+CD25+ T cells / Fiori, Agnese [VerfasserIn]; 1 January 2021 (Online-Ressource)
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