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Verfasst von:Kolbe, Ulrike [VerfasserIn]   i
 Yi, Buqing [VerfasserIn]   i
 Poth, Tanja [VerfasserIn]   i
 Saunders, Amy [VerfasserIn]   i
 Boutin, Sébastien [VerfasserIn]   i
 Dalpke, Alexander [VerfasserIn]   i
Titel:Early cytokine induction upon Pseudomonas aeruginosa infection in murine precision cut lung slices depends on sensing of bacterial viability
Verf.angabe:Ulrike Kolbe, Buqing Yi, Tanja Poth, Amy Saunders, Sébastien Boutin and Alexander H. Dalpke
E-Jahr:2020
Jahr:30 October 2020
Umfang:16 S.
Teil:volume:11
 year:2020
 elocationid:598636
 pages:1-16
 extent:16
Fussnoten:Gesehen am 10.06.2021
Titel Quelle:Enthalten in: Frontiers in immunology
Ort Quelle:Lausanne : Frontiers Media, 2010
Jahr Quelle:2020
Band/Heft Quelle:11(2020), Artikel-ID 598636, Seite 1-16
ISSN Quelle:1664-3224
Abstract:Breathing allows a multitude of airborne microbes and microbial compounds to access the lung. Constant exposure of the pulmonary microenvironment to immunogenic particles illustrates the need for proper control mechanisms ensuring the differentiation between threatening and harmless encounters. Discrimination between live and dead bacteria has been suggested to be such a mechanism. In this study, we performed infection studies of murine precision cut lung slices (PCLS) with live or heat-killed P. aeruginosa, in order to investigate the role of viability for induction of an innate immune response. We demonstrate that PCLS induce a robust transcriptomic rewiring upon infection with live but not heat-killed P. aeruginosa. Using mutants of the P. aeruginosa clinical isolate CHA, we show that the viability status of P. aeruginosa is assessed in PCLS by TLR5-independent sensing of flagellin and recognition of the type three secretion system. We further demonstrate that enhanced cytokine expression towards live P. aeruginosa is mediated by uptake of viable but not heat-killed bacteria. Finally, by using a combined approach of receptor blockage and genetically-modified PCLS we report a redundant involvement of MARCO and CD200R1 in the uptake of live P. aeruginosa in PCLS. Altogether, our results show that PCLS adapt the extent of cytokine expression to the viability status of P. aeruginosa by specifically internalizing live bacteria.
DOI:doi:10.3389/fimmu.2020.598636
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.3389/fimmu.2020.598636
 Volltext: https://www.frontiersin.org/articles/10.3389/fimmu.2020.598636/full
 DOI: https://doi.org/10.3389/fimmu.2020.598636
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:infection and immunity
 Innate immunity
 Live/dead discrimination
 Precision Cut Lung Slice
 Pseudomonas aeruginosa
K10plus-PPN:1760239488
Verknüpfungen:→ Zeitschrift

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