Cytokine/chemokine transcript profiles reflect mucosal inflammation in Crohn's disease

• Verfasst von: Stallmach, Andreas [VerfasserIn]
• Verfasst von: Giese, Thomas [VerfasserIn]
• Verfasst von: Schmidt, Carsten [VerfasserIn]
• Verfasst von: Ludwig, Bianca [VerfasserIn]
• Verfasst von: Mueller-Molaian, Ina [VerfasserIn]
• Verfasst von: Meuer, Stefan [VerfasserIn]
• Titel: Cytokine/chemokine transcript profiles reflect mucosal inflammation in Crohn's disease
• Verf.angabe: Andreas Stallmach, Thomas Giese, Carsten Schmidt, Bianca Ludwig, Ina Mueller-Molaian & Stefan C. Meuer
• E-Jahr: 2004
• Jahr: 07 November 2003
• Umfang: 8 S.
• Teil: volume:19
• Teil: year:2004
• Teil: number:4
• Teil: pages:308-315
• Teil: extent:8
• Fussnoten: Gesehen am 14.06.2021
• Titel Quelle: Enthalten in: International journal of colorectal disease
• Ort Quelle: Berlin : Springer, 1986
• Jahr Quelle: 2004
• Band/Heft Quelle: 19(2004), 4, Seite 308-315
• ISSN Quelle: 1432-1262
• DOI: doi:10.1007/s00384-003-0554-4
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Adolescent
• Sach-SW: Adult
• Sach-SW: Aged
• Sach-SW: Aged, 80 and over
• Sach-SW: Azathioprine
• Sach-SW: Case-Control Studies
• Sach-SW: Chemokines
• Sach-SW: Crohn Disease
• Sach-SW: Cytokines
• Sach-SW: Female
• Sach-SW: Gene Expression
• Sach-SW: Glucocorticoids
• Sach-SW: Humans
• Sach-SW: Immunosuppressive Agents
• Sach-SW: Intestinal Mucosa
• Sach-SW: Male
• Sach-SW: Middle Aged
• Sach-SW: Polymerase Chain Reaction
• Sach-SW: Predictive Value of Tests
• Sach-SW: Prednisolone
• Sach-SW: RNA, Messenger
• Sach-SW: Sensitivity and Specificity
• K10plus-PPN: 1760395471

Abstract

BACKGROUND AND AIMS: Immunoregulatory properties of cytokines may contribute to pathological immune reactions in inflammatory bowel disease. There is an urgent need for a simple and dependable means for quantitating inflammatory activity in mucosal biopsies and assessing relapse risk particularly in patients with active Crohn's disease (CD). PATIENTS AND METHODS: Cytokine and chemokine transcripts were quantified using real-time PCR in mucosal biopsy specimens from 70 patients with active inflammatory bowel disease (CD, n=45; ulcerative colitis n=25) and 16 patients with specific colitis (ischemic colitis, infectious colitis). Controls were 12 patients with noninflammatory conditions. CD patients with steroid-induced remission (n=20) were followed for up to 12 months. RESULTS: Compared to not-inflamed mucosa the vast majority of active CD tissue samples expressed significantly elevated transcript levels of IL-1beta, IL-8, IL-23, MRP-14, MIP2alpha, and MMP-1. Moreover, increased cytokine transcript levels were detected in both active ulcerative colitis and specific colitis. Importantly, TNF-alpha, IFN-gamma, CD40L, and IL-23 transcripts increased in active CD only. Transcript levels (MRP-14, IL-8, MMP-1, MIP2alpha) were correlated with clinical disease activity (CDAI) and endoscopic scoring indices. Medical treatment induced stable remission in 14 of 20 patients which was paralleled by a reduction in increased transcript levels. All six patients without normalization of MIP2alpha, MRP-14, TNF-alpha, and IL-1beta transcripts developed an early relapse (n=5) or chronic activity (n=1) during follow-up. CONCLUSION: Elevated proinflammatory cytokine transcripts in active CD may underlie disease reactivation and chronicity. Real-time PCR quantification is a simple and objective method for grading inflammation of intestinal mucosa and may be useful in identifying patients who would benefit from anti-inflammatory remission maintenance.