| Online-Ressource |
Verfasst von: | Schmidt, Carsten [VerfasserIn]  |
| Giese, Thomas [VerfasserIn]  |
| Hermann, Eva [VerfasserIn]  |
| Zeuzem, Stefan [VerfasserIn]  |
| Meuer, Stefan [VerfasserIn]  |
| Stallmach, Andreas [VerfasserIn]  |
Titel: | Predictive value of mucosal TNF-alpha transcripts in steroid-refractory Crohn's disease patients receiving intensive immunosuppressive therapy |
Verf.angabe: | Carsten Schmidt, Thomas Giese, Eva Hermann, Stefan Zeuzem, Stefan C. Meuer, and Andreas Stallmach |
Jahr: | 2007 |
Umfang: | 6 S. |
Teil: | volume:13 |
| year:2007 |
| number:1 |
| pages:65-70 |
| extent:6 |
Fussnoten: | Gesehen am 16.06.2021 |
Titel Quelle: | Enthalten in: Inflammatory bowel diseases |
Ort Quelle: | Oxford : Oxford University Press, 1995 |
Jahr Quelle: | 2007 |
Band/Heft Quelle: | 13(2007), 1, Seite 65-70 |
ISSN Quelle: | 1536-4844 |
Abstract: | BACKGROUND: Concentrations of proinflammatory cytokines are increased in the intestinal mucosa of patients with active Crohn's disease (CD). In a prospective study we investigated whether cytokines can predict long-term remission (>6 months) in patients with steroid-refractory CD receiving treatment with infliximab or cyclophosphamide, followed by azathioprine or methotrexate. METHODS: Cytokine transcripts were quantified using real-time polymerase chain reaction (PCR) in mucosal biopsies from 19 patients with active, steroid-refractory CD before and 8 weeks after initiation of therapy. Patients were treated with cyclophosphamide (monthly treatment of 750 mg cyclophosphamide intravenously) or infliximab (5 mg/kg body weight) and were followed until relapse of the disease. Statistical analysis was performed to identify predictive factors to discriminate between patients with or without long-term remission. RESULTS: Seventeen out of 19 patients achieved remission of the disease, two patients were nonresponders, while six out of 17 patients exhibited an early recurrence. Pretreatment TNF-alpha, IL-18, MRP-14, and IL-8 transcripts were significantly correlated with long-term remission. While several cytokines, most importantly MMP-1, determined after 8 weeks were able to predict patients achieving long-term remission, only a decrease of TNF-alpha levels after 8 weeks was predictive. Overall, statistical analysis identified lower pretreatment TNF-alpha levels as the strongest predictor of long-term remission among baseline variables. CONCLUSIONS: Quantification of mucosal TNF-alpha transcripts prior to therapy allows identification of patients achieving long-term remission upon immunosuppression with infliximab or cyclophosphamide. Real-time PCR might have considerable potential in the analysis of disease activity and subsequent clinical management of patients with immunosuppressive therapies. |
DOI: | doi:10.1002/ibd.20012 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://dx.doi.org/10.1002/ibd.20012 |
| DOI: https://doi.org/10.1002/ibd.20012 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | Adolescent |
| Adult |
| Antibodies, Monoclonal |
| Chemokines |
| Crohn Disease |
| Cyclophosphamide |
| Cytokines |
| Drug Resistance |
| Female |
| Gastrointestinal Agents |
| Glucocorticoids |
| Humans |
| Immunosuppressive Agents |
| Infliximab |
| Intestinal Mucosa |
| Male |
| Middle Aged |
| Prednisolone |
| Remission Induction |
| Tumor Necrosis Factor-alpha |
K10plus-PPN: | 1760544183 |
Verknüpfungen: | → Zeitschrift |
Predictive value of mucosal TNF-alpha transcripts in steroid-refractory Crohn's disease patients receiving intensive immunosuppressive therapy / Schmidt, Carsten [VerfasserIn]; 2007 (Online-Ressource)