Expression of interleukin-12-related cytokine transcripts in inflammatory bowel disease

• Verfasst von: Schmidt, Carsten [VerfasserIn]
• Verfasst von: Giese, Thomas [VerfasserIn]
• Verfasst von: Ludwig, Bianca [VerfasserIn]
• Verfasst von: Mueller-Molaian, Ina [VerfasserIn]
• Verfasst von: Marth, Thomas [VerfasserIn]
• Verfasst von: Zeuzem, Stefan [VerfasserIn]
• Verfasst von: Meuer, Stefan [VerfasserIn]
• Verfasst von: Stallmach, Andreas [VerfasserIn]
• Titel: Expression of interleukin-12-related cytokine transcripts in inflammatory bowel disease
• Titelzusatz: elevated interleukin-23p19 and interleukin-27p28 in Crohn's disease but not in ulcerative colitis
• Verf.angabe: Carsten Schmidt, Thomas Giese, Bianca Ludwig, Ina Mueller-Molaian, Thomas Marth, Stefan Zeuzem, Stefan C. Meuer, and Andreas Stallmach
• Jahr: 2005
• Umfang: 8 S.
• Teil: volume:11
• Teil: year:2005
• Teil: number:1
• Teil: pages:16-23
• Teil: extent:8
• Fussnoten: Gesehen am 16.06.2021
• Titel Quelle: Enthalten in: Inflammatory bowel diseases
• Ort Quelle: Oxford : Oxford University Press, 1995
• Jahr Quelle: 2005
• Band/Heft Quelle: 11(2005), 1, Seite 16-23
• ISSN Quelle: 1536-4844
• DOI: doi:10.1097/00054725-200501000-00003
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Adolescent
• Sach-SW: Adult
• Sach-SW: Aged
• Sach-SW: Aged, 80 and over
• Sach-SW: Case-Control Studies
• Sach-SW: Colitis, Ulcerative
• Sach-SW: Crohn Disease
• Sach-SW: Female
• Sach-SW: Humans
• Sach-SW: Interleukin-23
• Sach-SW: Interleukin-23 Subunit p19
• Sach-SW: Interleukins
• Sach-SW: Intestinal Mucosa
• Sach-SW: Male
• Sach-SW: Middle Aged
• Sach-SW: Reverse Transcriptase Polymerase Chain Reaction
• Sach-SW: RNA, Messenger
• Sach-SW: Transcription, Genetic
• Sach-SW: Up-Regulation
• K10plus-PPN: 1760545287

Abstract

BACKGROUND: It has been suggested that Crohn's disease (CD) is associated with an exaggerated T-helper 1 cytokine response manifested by increased production of interleukin (IL)-12. IL-12 is a heterodimeric protein comprising 2 disulfide-linked subunits designated p35 and p40. Recently, IL-12-related cytokines, IL-23 and IL-27, were described. Biologically active IL-23 is a heterodimer whose p40 subunit is identical to IL-12p40 whereas its p19 subunit is distantly related to IL-12p35. IL-27 consists of EBI3, an IL-12p40-related protein, and p28, a newly discovered IL-12p35-related polypeptide. AIM: We sought to determine whether mucosal expression of IL-23p19 and IL-27p28 transcripts correlate with the inflammatory activity in inflammatory bowel disease (IBD). PATIENTS/METHODS: Messenger RNA expression in colonic mucosa from patients with Crohn's disease (CD; n = 37) and ulcerative colitis (UC; n = 19), and in non-IBD control subjects (specific colitis [SC]; n = 16) and normal, nondiseased control patients (n = 12) was measured by reverse-transcribed real-time polymerase chain reaction. RESULTS: IL-23p19 was significantly increased in inflamed mucosa in CD (P = 0.0377) and to a lesser extent also in UC patients but not in SC patients. Elevation of IL-23p19 transcript levels in CD correlated with the severity of endoscopic lesions. IL-27p28 transcripts and EBI3 transcripts were significantly elevated only in active CD. DISCUSSION: IL-23p19, IL-27p28, and EBI3 transcripts are strongly up-regulated in CD. The stimulatory effects of these cytokines on naive T cells in addition to a strongly synergistic action with IL-12 to trigger interferon-gamma production may contribute to the perpetuation of the inflammatory process in patients with CD. Notably, increased expression of IL-23 and IL-27 transcripts in CD suggests a T helper 1-dominated immunologic function in this disease.