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Verfasst von:Brenner, David [VerfasserIn]   i
 Yılmaz, Rüstem [VerfasserIn]   i
 Müller, Kathrin [VerfasserIn]   i
 Grehl, Torsten [VerfasserIn]   i
 Petri, Susanne [VerfasserIn]   i
 Meyer, Thomas [VerfasserIn]   i
 Großkreutz, Julian [VerfasserIn]   i
 Weydt, Patrick [VerfasserIn]   i
 Ruf, Wolfgang [VerfasserIn]   i
 Neuwirth, Christoph [VerfasserIn]   i
 Weber, Markus [VerfasserIn]   i
 Pinto, Susana [VerfasserIn]   i
 Claeys, Kristl G. [VerfasserIn]   i
 Schrank, Berthold [VerfasserIn]   i
 Jordan, Berit [VerfasserIn]   i
 Knehr, Antje [VerfasserIn]   i
 Günther, Kornelia [VerfasserIn]   i
 Hübers, Annemarie [VerfasserIn]   i
 Zeller, Daniel [VerfasserIn]   i
 Kubisch, Christian [VerfasserIn]   i
 Jablonka, Sibylle [VerfasserIn]   i
 Sendtner, Michael [VerfasserIn]   i
 Klopstock, Thomas [VerfasserIn]   i
 de Carvalho, Mamede [VerfasserIn]   i
 Sperfeld, Anne [VerfasserIn]   i
 Borck, Guntram [VerfasserIn]   i
 Volk, Alexander E. [VerfasserIn]   i
 Dorst, Johannes [VerfasserIn]   i
 Weis, Joachim [VerfasserIn]   i
 Otto, Markus [VerfasserIn]   i
 Schuster, Joachim [VerfasserIn]   i
 Del Tredici, Kelly [VerfasserIn]   i
 Braak, Heiko [VerfasserIn]   i
 Danzer, Karin M. [VerfasserIn]   i
 Freischmidt, Axel [VerfasserIn]   i
 Meitinger, Thomas [VerfasserIn]   i
 Strom, Tim M. [VerfasserIn]   i
 Ludolph, Albert C. [VerfasserIn]   i
 Andersen, Peter M. [VerfasserIn]   i
 Weishaupt, Jochen H. [VerfasserIn]   i
Titel:Hot-spot KIF5A mutations cause familial ALS
Verf.angabe:David Brenner, Rüstem Yilmaz, Kathrin Müller, Torsten Grehl, Susanne Petri, Thomas Meyer, Julian Grosskreutz, Patrick Weydt, Wolfgang Ruf, Christoph Neuwirth, Markus Weber, Susana Pinto, Kristl G. Claeys, Berthold Schrank, Berit Jordan, Antje Knehr, Kornelia Günther, Annemarie Hübers, Daniel Zeller, Christian Kubisch, Sibylle Jablonka, Michael Sendtner, Thomas Klopstock, Mamede de Carvalho, Anne Sperfeld, Guntram Borck, Alexander E. Volk, Johannes Dorst, Joachim Weis, Markus Otto, Joachim Schuster, Kelly Del Tredici, Heiko Braak, Karin M. Danzer, Axel Freischmidt, Thomas Meitinger, Tim M. Strom, Albert C. Ludolph, Peter M. Andersen and Jochen H. Weishaupt
E-Jahr:2018
Jahr:12 January 2018
Umfang:10 S.
Fussnoten:Gesehen am 16.06.2021
Titel Quelle:Enthalten in: Brain
Ort Quelle:Oxford : Oxford Univ. Press, 1878
Jahr Quelle:2018
Band/Heft Quelle:141(2018), 3 vom: März, Seite 688-697
ISSN Quelle:1460-2156
Abstract:Heterozygous missense mutations in the N-terminal motor or coiled-coil domains of the kinesin family member 5A (KIF5A) gene cause monogenic spastic paraplegia (HSP10) and Charcot-Marie-Tooth disease type 2 (CMT2). Moreover, heterozygous de novo frame-shift mutations in the C-terminal domain of KIF5A are associated with neonatal intractable myoclonus, a neurodevelopmental syndrome. These findings, together with the observation that many of the disease genes associated with amyotrophic lateral sclerosis disrupt cytoskeletal function and intracellular transport, led us to hypothesize that mutations in KIF5A are also a cause of amyotrophic lateral sclerosis. Using whole exome sequencing followed by rare variant analysis of 426 patients with familial amyotrophic lateral sclerosis and 6137 control subjects, we detected an enrichment of KIF5A splice-site mutations in amyotrophic lateral sclerosis (2/426 compared to 0/6137 in controls; P = 4.2 × 10-3), both located in a hot-spot in the C-terminus of the protein and predicted to affect splicing exon 27. We additionally show co-segregation with amyotrophic lateral sclerosis of two canonical splice-site mutations in two families. Investigation of lymphoblast cell lines from patients with KIF5A splice-site mutations revealed the loss of mutant RNA expression and suggested haploinsufficiency as the most probable underlying molecular mechanism. Furthermore, mRNA sequencing of a rare non-synonymous missense mutation (predicting p.Arg1007Gly) located in the C-terminus of the protein shortly upstream of the splice donor of exon 27 revealed defective KIF5A pre-mRNA splicing in respective patient-derived cell lines owing to abrogation of the donor site. Finally, the non-synonymous single nucleotide variant rs113247976 (minor allele frequency = 1.00% in controls, n = 6137), also located in the C-terminal region [p.(Pro986Leu) in exon 26], was significantly enriched in familial amyotrophic lateral sclerosis patients (minor allele frequency = 3.40%; P = 1.28 × 10-7). Our study demonstrates that mutations located specifically in a C-terminal hotspot of KIF5A can cause a classical amyotrophic lateral sclerosis phenotype, and underline the involvement of intracellular transport processes in amyotrophic lateral sclerosis pathogenesis.
DOI:doi:10.1093/brain/awx370
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

DOI: https://doi.org/10.1093/brain/awx370
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Adult
 Aged
 Amyotrophic Lateral Sclerosis
 DNA Mutational Analysis
 Family Health
 Female
 Genetic Association Studies
 Humans
 Kinesin
 Lymphocytes
 Male
 Middle Aged
 Mutation
 RNA, Messenger
K10plus-PPN:1760576204
Verknüpfungen:→ Zeitschrift

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