| |  Online-Ressource |
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| Verfasst von: | Zipplies, Johanna Katrin [VerfasserIn]  |
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| | Kirschfink, Michael [VerfasserIn] |
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| | Amann, Barbara [VerfasserIn] |
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| | Hauck, Stefanie M. [VerfasserIn] |
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| | Stangassinger, Manfred [VerfasserIn] |
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| | Deeg, Cornelia A. [VerfasserIn] |
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| Titel: | Complement factor B expression profile in a spontaneous uveitis model |
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| Verf.angabe: | Johanna K. Zipplies, Michael Kirschfink, Barbara Amann, Stefanie M. Hauck, Manfred Stangassinger, Cornelia A. Deeg |
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| E-Jahr: | 2010 |
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| Jahr: | 1 March 2010 |
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| Umfang: | 7 S. |
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| Teil: | volume:215 |
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| | year:2010 |
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| | number:12 |
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| | pages:949-955 |
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| | extent:7 |
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| Fussnoten: | Gesehen am 18.06.2021 |
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| Titel Quelle: | Enthalten in: Immunobiology |
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| Ort Quelle: | München : Elsevier, 1979 |
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| Jahr Quelle: | 2010 |
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| Band/Heft Quelle: | 215(2010), 12, Seite 949-955 |
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| ISSN Quelle: | 1878-3279 |
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| Abstract: | Equine recurrent uveitis serves as a spontaneous model for human autoimmune uveitis. Unpredictable relapses and ongoing inflammation in the eyes of diseased horses as well as in humans lead to destruction of the retina and finally result in blindness. However, the molecular mechanisms leading to inflammation and retinal degeneration are not well understood. An initial screening for differentially regulated proteins in sera of uveitic cases compared to healthy controls revealed an increase of the alternative pathway complement component factor B in ERU cases. To determine the activation status of the complement system, sera were subsequently examined for complement split products. We could demonstrate a significant higher concentration of the activation products B/Ba, B/Bb, Bb neoantigen, iC3b and C3d in uveitic condition compared to healthy controls, whereas for C5b-9 no differences were detected. Additionally, we investigated complement activation directly in the retina by immunohistochemistry, since it is the main target organ of this autoimmune disease. Interestingly, infiltrating cells co-expressed activated factor Bb neoantigen, complement split product C3d as well as CD68, a macrophage marker. In this study, we could demonstrate activation of the complement system both systemically as well as in the eye, the target organ of spontaneous recurrent uveitis. Based on these novel findings, we postulate a novel role for macrophages in connection with complement synthesis at the site of inflammation. |
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| DOI: | doi:10.1016/j.imbio.2010.02.003 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://dx.doi.org/10.1016/j.imbio.2010.02.003 |
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| | Volltext: https://www.sciencedirect.com/science/article/abs/pii/S0171298510000148 |
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| | DOI: https://doi.org/10.1016/j.imbio.2010.02.003 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Animals |
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| | Antigens, CD |
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| | Antigens, Differentiation, Myelomonocytic |
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| | Autoimmune Diseases |
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| | Complement Activation |
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| | Complement C3b |
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| | Complement C3d |
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| | Complement Factor B |
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| | Complement Membrane Attack Complex |
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| | Disease Models, Animal |
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| | Electrophoresis, Gel, Two-Dimensional |
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| | Female |
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| | Horse Diseases |
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| | Horses |
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| | Humans |
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| | Immunohistochemistry |
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| | Macrophages |
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| | Male |
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| | Retina |
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| | Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
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| | Uveitis |
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| K10plus-PPN: | 1760867888 |
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| Verknüpfungen: | → Zeitschrift |
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Complement factor B expression profile in a spontaneous uveitis model / Zipplies, Johanna Katrin [VerfasserIn]; 1 March 2010 (Online-Ressource)
