| |  Online-Ressource |
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| Verfasst von: | Schilling, Maximilian Thomas [VerfasserIn]  |
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| | Prusty, Archana B. [VerfasserIn] |
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| | Boysen, Björn [VerfasserIn] |
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| | Oppermann, Felix Sebastian [VerfasserIn] |
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| | Riedel, Yannick L. [VerfasserIn] |
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| | Husedzinovic, Alma [VerfasserIn] |
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| | Rasouli, Homa [VerfasserIn] |
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| | König, Angelika [VerfasserIn] |
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| | Ramanathan, Pradhipa [VerfasserIn] |
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| | Reymann, Jürgen [VerfasserIn] |
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| | Erfle, Holger [VerfasserIn] |
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| | Daub, Henrik [VerfasserIn] |
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| | Fischer, Utz [VerfasserIn] |
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| | Gruss, Oliver [VerfasserIn] |
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| Titel: | TOR signaling regulates liquid phase separation of the SMN complex governing snRNP biogenesis |
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| Verf.angabe: | Maximilian Schilling, Archana B. Prusty, Björn Boysen, Felix S. Oppermann, Yannick L. Riedel, Alma Husedzinovic, Homa Rasouli, Angelika König, Pradhipa Ramanathan, Jürgen Reymann, Holger Erfle, Henrik Daub, Utz Fischer, and Oliver J. Gruss |
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| E-Jahr: | 2021 |
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| Jahr: | 22 June 2021 |
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| Umfang: | 20 S. |
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| Teil: | volume:35 |
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| | year:2021 |
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| | number:12 |
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| | elocationid:109277 |
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| | extent:20 |
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| Fussnoten: | Gesehen am 23.06.2021 |
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| Titel Quelle: | Enthalten in: Cell reports |
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| Ort Quelle: | Maryland Heights, MO : Cell Press, 2012 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 35(2021), 12, Artikel-ID 109277 |
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| ISSN Quelle: | 2211-1247 |
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| Abstract: | The activity of the SMN complex in promoting the assembly of pre-mRNA processing UsnRNPs correlates with condensation of the complex in nuclear Cajal bodies. While mechanistic details of its activity have been elucidated, the molecular basis for condensation remains unclear. High SMN complex phosphorylation suggests extensive regulation. Here, we report on systematic siRNA-based screening for modulators of the capacity of SMN to condense in Cajal bodies and identify mTOR and ribosomal protein S6 kinase β-1 as key regulators. Proteomic analysis reveals TOR-dependent phosphorylations in SMN complex subunits. Using stably expressed or optogenetically controlled phospho mutants, we demonstrate that serine 49 and 63 phosphorylation of human SMN controls the capacity of the complex to condense in Cajal bodies via liquid-liquid phase separation. Our findings link SMN complex condensation and UsnRNP biogenesis to cellular energy levels and suggest modulation of TOR signaling as a rational concept for therapy of the SMN-linked neuromuscular disorder spinal muscular atrophy. |
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| DOI: | doi:10.1016/j.celrep.2021.109277 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.celrep.2021.109277 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S2211124721006446 |
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| | DOI: https://doi.org/10.1016/j.celrep.2021.109277 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | assembly of ribonucleoprotein complexes |
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| | Cajal body |
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| | LLPS |
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| | mTOR |
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| | optogenetics |
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| | phase separation |
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| | ribosomal protein S6-kinase β-1 (RPS6KB1) |
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| | SMA |
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| | SMN |
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| | snRNP |
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| K10plus-PPN: | 1761083716 |
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| Verknüpfungen: | → Zeitschrift |
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TOR signaling regulates liquid phase separation of the SMN complex governing snRNP biogenesis / Schilling, Maximilian Thomas [VerfasserIn]; 22 June 2021 (Online-Ressource)
