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| Verfasst von: | Gutscher, Marcus [VerfasserIn]  |
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| | Pauleau, Anne-Laure [VerfasserIn] |
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| | Marty, Laurent [VerfasserIn] |
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| | Brach, Thorsten [VerfasserIn] |
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| | Wabnitz, Guido H. [VerfasserIn] |
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| | Samstag, Yvonne [VerfasserIn] |
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| | Meyer, Andreas [VerfasserIn] |
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| | Dick, Tobias P. [VerfasserIn] |
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| Titel: | Real-time imaging of the intracellular glutathione redox potential |
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| Verf.angabe: | Marcus Gutscher, Anne-Laure Pauleau, Laurent Marty, Thorsten Brach, Guido H. Wabnitz, Yvonne Samstag, Andreas J. Meyer & Tobias P. Dick |
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| Jahr: | 2008 |
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| Umfang: | 7 S. |
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| Fussnoten: | Gesehen am 25.06.2021 |
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| Titel Quelle: | Enthalten in: Nature methods |
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| Ort Quelle: | London [u.a.] : Nature Publishing Group, 2004 |
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| Jahr Quelle: | 2008 |
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| Band/Heft Quelle: | 5(2008), 6, Seite 553-559 |
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| ISSN Quelle: | 1548-7105 |
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| Abstract: | Dynamic analysis of redox-based processes in living cells is now restricted by the lack of appropriate redox biosensors. Conventional redox-sensitive GFPs (roGFPs) are limited by undefined specificity and slow response to changes in redox potential. In this study we demonstrate that the fusion of human glutaredoxin-1 (Grx1) to roGFP2 facilitates specific real-time equilibration between the sensor protein and the glutathione redox couple. The Grx1-roGFP2 fusion protein allowed dynamic live imaging of the glutathione redox potential (E(GSH)) in different cellular compartments with high sensitivity and temporal resolution. The biosensor detected nanomolar changes in oxidized glutathione (GSSG) against a backdrop of millimolar reduced glutathione (GSH) on a scale of seconds to minutes. It facilitated the observation of redox changes associated with growth factor availability, cell density, mitochondrial depolarization, respiratory burst activity and immune receptor stimulation. |
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| DOI: | doi:10.1038/nmeth.1212 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://dx.doi.org/10.1038/nmeth.1212 |
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| | Volltext: https://www.nature.com/articles/nmeth.1212 |
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| | DOI: https://doi.org/10.1038/nmeth.1212 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Apoptosis |
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| | Biosensing Techniques |
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| | Biotechnology |
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| | Disulfides |
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| | Glutaredoxins |
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| | Glutathione |
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| | Green Fluorescent Proteins |
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| | HeLa Cells |
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| | Humans |
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| | Mitochondria |
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| | Oxidation-Reduction |
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| | Oxygen |
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| | Recombinant Fusion Proteins |
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| | Sensitivity and Specificity |
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| | Thioredoxins |
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| K10plus-PPN: | 1761255878 |
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| Verknüpfungen: | → Zeitschrift |
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Real-time imaging of the intracellular glutathione redox potential / Gutscher, Marcus [VerfasserIn]; 2008 (Online-Ressource)
