Fine tuning and efficient T cell activation with stimulatory aCD3 nanoarrays

• Verfasst von: Matić, Jovana [VerfasserIn]
• Verfasst von: Deeg, Janosch A. [VerfasserIn]
• Verfasst von: Scheffold, Alexander [VerfasserIn]
• Verfasst von: Goldstein, Itamar [VerfasserIn]
• Verfasst von: Spatz, Joachim P. [VerfasserIn]
• Titel: Fine tuning and efficient T cell activation with stimulatory aCD3 nanoarrays
• Verf.angabe: Jovana Matic, Janosch Deeg, Alexander Scheffold, Itamar Goldstein, and Joachim P. Spatz
• E-Jahr: 2013
• Jahr: October 10, 2013
• Umfang: 8 S.
• Teil: volume:13
• Teil: year:2013
• Teil: number:11
• Teil: pages:5090-5097
• Teil: extent:8
• Fussnoten: Gesehen am 25.06.2021
• Titel Quelle: Enthalten in: Nano letters
• Ort Quelle: Washington, DC : ACS Publ., 2001
• Jahr Quelle: 2013
• Band/Heft Quelle: 13(2013), 11, Seite 5090-5097
• ISSN Quelle: 1530-6992
• DOI: doi:10.1021/nl4022623
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1761308688

Abstract

Anti-CD3 (aCD3) nanoarrays fabricated by self-assembled nanopatterning combined with site-directed protein immobilization techniques represent a novel T cell stimulatory platform that allows tight control over ligand orientation and surface density. Here, we show that activation of primary human CD4+ T cells, defined by CD69 upregulation, IL-2 production and cell proliferation, correlates with aCD3 density on nanoarrays. Immobilization of aCD3 through nanopatterning had two effects: cell activation was significantly higher on these surfaces than on aCD3-coated plastics and allowed unprecedented fine-tuning of T cell response.