Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Strzeszewska-Potyrała, Anna [VerfasserIn]   i
 Staniak, Karolina [VerfasserIn]   i
 Czarnecka-Herok, Joanna [VerfasserIn]   i
 Rafiee, Mahmoud-Reza [VerfasserIn]   i
 Herok, Marcin [VerfasserIn]   i
 Mosieniak, Grażyna [VerfasserIn]   i
 Krijgsveld, Jeroen [VerfasserIn]   i
 Sikora, Ewa [VerfasserIn]   i
Titel:Chromatin-directed proteomics identifies ZNF84 as a p53-independent regulator of p21 in genotoxic stress response
Verf.angabe:Anna Strzeszewska-Potyrała, Karolina Staniak, Joanna Czarnecka-Herok, Mahmoud-Reza Rafiee, Marcin Herok, Grażyna Mosieniak, Jeroen Krijgsveld and Ewa Sikora
E-Jahr:2021
Jahr:7 April 2021
Umfang:24 S.
Teil:volume:13
 year:2021
 number:9
 elocationid:2115
 pages:1-24
 extent:24
Fussnoten:Gesehen am 28.06.2021
Titel Quelle:Enthalten in: Cancers
Ort Quelle:Basel : MDPI, 2009
Jahr Quelle:2021
Band/Heft Quelle:13(2021), 9, Artikel-ID 2115, Seite 1-24
ISSN Quelle:2072-6694
Abstract:The p21WAF1/Cip1 protein, encoded by CDKN1A, plays a vital role in senescence, and its transcriptional control by the tumour suppressor p53 is well-established. However, p21 can also be regulated in a p53-independent manner, by mechanisms that still remain less understood. We aimed to expand the knowledge about p53-independent senescence by looking for novel players involved in CDKN1A regulation. We used a chromatin-directed proteomic approach and identified ZNF84 as a novel regulator of p21 in various p53-deficient cell lines treated with cytostatic dose of doxorubicin. Knock-down of ZNF84, an as-yet un-characterized protein, inhibited p21 gene and protein expression in response to doxorubicin, it attenuated senescence and was associated with enhanced proliferation, indicating that ZNF84-deficiency can favor senescence bypass. ZNF84 deficiency was also associated with transcriptomic changes in genes governing various cancer-relevant processes e.g., mitosis. In cells with ZNF84 knock-down we discovered significantly lower level of H2AX Ser139 phosphorylation (γH2AX), which is triggered by DNA double strand breaks. Intriguingly, we observed a reverse correlation between the level of ZNF84 expression and survival rate of colon cancer patients. In conclusion, ZNF84, whose function was previously not recognized, was identified here as a critical p53-independent regulator of senescence, opening possibilities for its targeting in novel therapies of p53-null cancers.
DOI:doi:10.3390/cancers13092115
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.3390/cancers13092115
 Volltext: https://www.mdpi.com/2072-6694/13/9/2115
 DOI: https://doi.org/10.3390/cancers13092115
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:<i>CDKN1A</i>
 chemotherapy
 chromatin pulldown
 genotoxic stress
 mass spectrometry
 p21
 senescence
 ZNF84
K10plus-PPN:176132134X
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68753043   QR-Code
zum Seitenanfang