Biofilm growth on implants

• Verfasst von: Wagner, Christof [VerfasserIn]
• Verfasst von: Aytaç, Sâra [VerfasserIn]
• Verfasst von: Hänsch, Gertrud Maria [VerfasserIn]
• Titel: Biofilm growth on implants
• Titelzusatz: bacteria prefer plasma coats
• Verf.angabe: Christof Wagner, Sara Aytac, G. Maria Hänsch
• E-Jahr: 2011
• Jahr: November 17, 2011
• Umfang: 7 S.
• Fussnoten: Gesehen am 30.06.2021
• Titel Quelle: Enthalten in: The international journal of artificial organs
• Ort Quelle: Thousand Oaks, CA : Sage Publishing, 1978
• Jahr Quelle: 2011
• Band/Heft Quelle: 34(2011), 9, Seite 811-817
• ISSN Quelle: 1724-6040
• DOI: doi:10.5301/ijao.5000061
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Bacterial Adhesion
• Sach-SW: Biofilms
• Sach-SW: Blood Proteins
• Sach-SW: Cell Line, Tumor
• Sach-SW: Coated Materials, Biocompatible
• Sach-SW: Humans
• Sach-SW: Joint Prosthesis
• Sach-SW: Polystyrenes
• Sach-SW: Prosthesis Design
• Sach-SW: Pseudomonas aeruginosa
• Sach-SW: Staphylococcus aureus
• Sach-SW: Surface Properties
• Sach-SW: Time Factors
• Sach-SW: Titanium
• K10plus-PPN: 1761623737

Abstract

PURPOSE: Bacterial biofilm formation on prostheses or devices used for osteosynthesis is increasingly recognized as cause of persistent infections, an entity known as implant-associated posttraumatic osteomyelitis. Biofilm formation is a very complex, multistep process with adhesion as the first and decisive step. The most prevalent pathogens found are staphylococci species, especially S. aureus, presumably due to a preference to non-biological materials, such as metal. Adherence is influenced by several factors, including the microenvironment, in which blood proteins from serum or plasma might influence adhesion and maybe biofilm formation. The aim of the present study was to test and to compare adherence of S. aureus and P. aeruginosa to different biological and non-biological surfaces in vitro. The question was addressed if coating of the surface by plasma or serum proteins influences bacterial adherence. METHODS: Adherence of radiolabeled bacteria to different surfaces in the presence or absence or serum/plasma proteins was measured over time. RESULTS: When testing adherence of S. aureus to plastic, titanium or to monolayers of epithelial cells (A549) or fibroblasts (Colo800) a clear-cut preference for non-biological surfaces, especially for titanium was seen. Using P. aeruginosa species a similar pattern without a significant difference was revealed. When mimicking the in vivo situation by pre-coating of titanium with human serum or plasma adherence was increased, especially when titanium was coated ("opsonized") by plasma. CONCLUSIONS: Bacterial adherence to surfaces is determined by a variety of factors such as temperature, the presence of nutrients, the absence of host defense systems and the configuration of the covered surface. In vivo, adherence to non-biological surfaces is also influenced by the microenvironment, especially plasma proteins, promoting biofilm formation.