| |  Online-Ressource |
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| Verfasst von: | Daugelavičienė, Neringa [VerfasserIn]  |
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| | Grigaitis, Pranas [VerfasserIn] |
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| | Gasiule, Liepa [VerfasserIn] |
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| | Dabkeviciene, Daiva [VerfasserIn] |
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| | Neniskyte, Urte [VerfasserIn] |
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| | Sasnauskiene, Ausra [VerfasserIn] |
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| Titel: | Lysosome-targeted photodynamic treatment induces primary keratinocyte differentiation |
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| Verf.angabe: | Neringa Daugelaviciene, Pranas Grigaitis, Liepa Gasiule, Daiva Dabkeviciene, Urte Neniskyte, Ausra Sasnauskiene |
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| E-Jahr: | 2021 |
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| Jahr: | May 2021 |
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| Umfang: | 12 S. |
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| Teil: | volume:218 |
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| | year:2021 |
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| | elocationid:112183 |
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| | pages:1-12 |
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| | extent:12 |
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| Fussnoten: | Gesehen am 07.07.2021 |
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| Titel Quelle: | Enthalten in: Journal of photochemistry and photobiology / B |
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| Ort Quelle: | New York, NY [u.a.] : Elsevier, 1987 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 218(2021), Artikel-ID 112183, Seite 1-12 |
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| ISSN Quelle: | 1873-2682 |
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| Abstract: | Photodynamic therapy is an attractive technique for various skin tumors and non-cancerous skin lesions. However, while the aim of photodynamic therapy is to target and damage only the malignant cells, it unavoidably affects some of the healthy cells surrounding the tumor as well. However, data on the effects of PDT to normal cells are scarce, and the characterization of the pathways activated after the photodamage of normal cells may help to improve clinical photodynamic therapy. In our study, primary human epidermal keratinocytes were used to evaluate photodynamic treatment effects of photosensitizers with different subcellular localization. We compared the response of keratinocytes to lysosomal photodamage induced by phthalocyanines, aluminum phthalocyanine disulfonate (AlPcS2a) or aluminum phthalocyanine tetrasulfonate (AlPcS4), and cellular membrane photodamage by m-tetra(3-hydroxyphenyl)-chlorin (mTHPC). Our data showed that mTHPC-PDT promoted autophagic flux, whereas lysosomal photodamage induced by aluminum phthalocyanines evoked differentiation and apoptosis. Photodamage by AlPcS2a, which is targeted to lysosomal membranes, induced keratinocyte differentiation and apoptosis more efficiently than AlPcS4, which is targeted to lysosomal lumen. Computational analysis of the interplay between these molecular pathways revealed that keratin 10 is the coordinating molecular hub of primary keratinocyte differentiation, apoptosis and autophagy. |
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| DOI: | doi:10.1016/j.jphotobiol.2021.112183 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.jphotobiol.2021.112183 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S1011134421000610 |
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| | DOI: https://doi.org/10.1016/j.jphotobiol.2021.112183 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | AlPcS |
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| | Keratin 10 |
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| | Keratinocyte differentiation |
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| | Lysosome |
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| | mTHPC |
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| | Photodynamic treatment |
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| | Primary human epidermal keratinocytes |
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| K10plus-PPN: | 1762301083 |
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| Verknüpfungen: | → Zeitschrift |
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Lysosome-targeted photodynamic treatment induces primary keratinocyte differentiation / Daugelavičienė, Neringa [VerfasserIn]; May 2021 (Online-Ressource)
