| |  Online-Ressource |
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| Verfasst von: | Müller, Thorsten G. [VerfasserIn]  |
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| | Zila, Vojtech [VerfasserIn] |
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| | Peters, Kyra [VerfasserIn] |
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| | Schifferdecker, Sandra [VerfasserIn] |
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| | Stanić, Mia [VerfasserIn] |
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| | Lucic, Bojana [VerfasserIn] |
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| | Laketa, Vibor [VerfasserIn] |
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| | Lusic, Marina [VerfasserIn] |
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| | Müller, Barbara [VerfasserIn] |
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| | Kräusslich, Hans-Georg [VerfasserIn] |
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| Titel: | HIV-1 uncoating by release of viral cDNA from capsid-like structures in the nucleus of infected cells |
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| Verf.angabe: | Thorsten G Müller, Vojtech Zila, Kyra Peters, Sandra Schifferdecker, Mia Stanic, Bojana Lucic, Vibor Laketa, Marina Lusic, Barbara Müller, Hans-Georg Kräusslich |
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| E-Jahr: | 2021 |
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| Jahr: | Apr 27, 2021 |
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| Umfang: | 32 S. |
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| Teil: | volume:10 |
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| | year:2021 |
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| | elocationid:e64776 |
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| | pages:1-32 |
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| | extent:32 |
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| Fussnoten: | Gesehen am 07.07.2021 |
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| Titel Quelle: | Enthalten in: eLife |
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| Ort Quelle: | Cambridge : eLife Sciences Publications, 2012 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 10(2021), Artikel-ID e64776, Seite 1-32 |
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| ISSN Quelle: | 2050-084X |
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| Abstract: | HIV-1 replication commences inside the cone-shaped viral capsid, but timing, localization, and mechanism of uncoating are under debate. We adapted a strategy to visualize individual reverse-transcribed HIV-1 cDNA molecules and their association with viral and cellular proteins using fluorescence and correlative-light-and-electron-microscopy (CLEM). We specifically detected HIV-1 cDNA inside nuclei, but not in the cytoplasm. Nuclear cDNA initially co-localized with a fluorescent integrase fusion (IN-FP) and the viral CA (capsid) protein, but cDNA-punctae separated from IN-FP/CA over time. This phenotype was conserved in primary HIV-1 target cells, with nuclear HIV-1 complexes exhibiting strong CA-signals in all cell types. CLEM revealed cone-shaped HIV-1 capsid-like structures and apparently broken capsid-remnants at the position of IN-FP signals and elongated chromatin-like structures in the position of viral cDNA punctae lacking IN-FP. Our data argue for nuclear uncoating by physical disruption rather than cooperative disassembly of the CA-lattice, followed by physical separation from the pre-integration complex. |
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| DOI: | doi:10.7554/eLife.64776 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.7554/eLife.64776 |
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| | DOI: https://doi.org/10.7554/eLife.64776 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | CLEM |
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| | DNA labeling |
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| | HIV-1 |
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| | live cell imaging |
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| | reverse transcription |
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| | uncoating |
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| K10plus-PPN: | 1762324822 |
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| Verknüpfungen: | → Zeitschrift |
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HIV-1 uncoating by release of viral cDNA from capsid-like structures in the nucleus of infected cells / Müller, Thorsten G. [VerfasserIn]; Apr 27, 2021 (Online-Ressource)
