| |  Online-Ressource |
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| Verfasst von: | Hesse, Julia [VerfasserIn]  |
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| | Owenier, Christoph [VerfasserIn] |
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| | Lautwein, Tobias [VerfasserIn] |
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| | Zalfen, Ria [VerfasserIn] |
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| | Weber, Jonas F [VerfasserIn] |
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| | Ding, Zhaoping [VerfasserIn] |
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| | Alter, Christina [VerfasserIn] |
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| | Lang, Alexander [VerfasserIn] |
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| | Grandoch, Maria [VerfasserIn] |
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| | Gerdes, Norbert [VerfasserIn] |
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| | Fischer, Jens W [VerfasserIn] |
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| | Klau, Gunnar W [VerfasserIn] |
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| | Dieterich, Christoph [VerfasserIn] |
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| | Köhrer, Karl [VerfasserIn] |
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| | Schrader, Jürgen [VerfasserIn] |
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| Titel: | Single-cell transcriptomics defines heterogeneity of epicardial cells and fibroblasts within the infarcted murine heart |
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| Verf.angabe: | Julia Hesse, Christoph Owenier, Tobias Lautwein, Ria Zalfen, Jonas F Weber, Zhaoping Ding, Christina Alter, Alexander Lang, Maria Grandoch, Norbert Gerdes, Jens W Fischer, Gunnar W Klau, Christoph Dieterich, Karl Köhrer, Jürgen Schrader |
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| E-Jahr: | 2021 |
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| Jahr: | 21 June 2021 |
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| Umfang: | 21 S. |
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| Teil: | volume:10 |
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| | year:2021 |
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| | elocationid:e65921 |
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| | pages:1-21 |
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| | extent:21 |
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| Fussnoten: | Gesehen am 16.07.2021 |
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| Titel Quelle: | Enthalten in: eLife |
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| Ort Quelle: | Cambridge : eLife Sciences Publications, 2012 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 10(2021), Artikel-ID e65921, Seite 1-21 |
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| ISSN Quelle: | 2050-084X |
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| Abstract: | In the adult heart, the epicardium becomes activated after injury, contributing to cardiac healing by secretion of paracrine factors. Here, we analyzed by single-cell RNA sequencing combined with RNA in situ hybridization and lineage tracing of Wilms tumor protein 1-positive (WT1+) cells, the cellular composition, location, and hierarchy of epicardial stromal cells (EpiSC) in comparison to activated myocardial fibroblasts/stromal cells in infarcted mouse hearts. We identified 11 transcriptionally distinct EpiSC populations, which can be classified into three groups, each containing a cluster of proliferating cells. Two groups expressed cardiac specification markers and sarcomeric proteins suggestive of cardiomyogenic potential. Transcripts of hypoxia-inducible factor (HIF)-1α and HIF-responsive genes were enriched in EpiSC consistent with an epicardial hypoxic niche. Expression of paracrine factors was not limited to WT1+ cells but was a general feature of activated cardiac stromal cells. Our findings provide the cellular framework by which myocardial ischemia may trigger in EpiSC the formation of cardioprotective/regenerative responses. |
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| DOI: | doi:10.7554/eLife.65921 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.7554/eLife.65921 |
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| | DOI: https://doi.org/10.7554/eLife.65921 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | cardiac fibroblasts |
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| | epicardium |
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| | myocardial infarction |
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| | ScRNAseq |
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| | transcriptomics |
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| K10plus-PPN: | 1763131661 |
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| Verknüpfungen: | → Zeitschrift |
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Single-cell transcriptomics defines heterogeneity of epicardial cells and fibroblasts within the infarcted murine heart / Hesse, Julia [VerfasserIn]; 21 June 2021 (Online-Ressource)
