| |  Online-Ressource |
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| Verfasst von: | Song, Jae-Eun [VerfasserIn]  |
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| | Alves, Tiago C. [VerfasserIn] |
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| | Stutz, Bernardo [VerfasserIn] |
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| | Šestan-Peša, Matija [VerfasserIn] |
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| | Kilian, Nicole [VerfasserIn] |
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| | Jin, Sungho [VerfasserIn] |
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| | Diano, Sabrina [VerfasserIn] |
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| | Kibbey, Richard G. [VerfasserIn] |
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| | Horvath, Tamas L. [VerfasserIn] |
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| Titel: | Mitochondrial fission governed by Drp1 regulates exogenous fatty acid usage and storage in hela cells |
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| Verf.angabe: | Jae-Eun Song, Tiago C. Alves, Bernardo Stutz, Matija Šestan-Peša, Nicole Kilian, Sungho Jin, Sabrina Diano, Richard G. Kibbey and Tamas L. Horvath |
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| E-Jahr: | 2021 |
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| Jahr: | 18 May 2021 |
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| Umfang: | 15 S. |
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| Teil: | volume:11 |
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| | year:2021 |
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| | number:5 |
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| | elocationid:322 |
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| | pages:1-15 |
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| | extent:15 |
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| Fussnoten: | Gesehen am 28.07.2021 |
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| Titel Quelle: | Enthalten in: Metabolites |
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| Ort Quelle: | Basel : MDPI, 2011 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 11(2021), 5, Artikel-ID 322, Seite 1-15 |
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| ISSN Quelle: | 2218-1989 |
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| Abstract: | In the presence of high abundance of exogenous fatty acids, cells either store fatty acids in lipid droplets or oxidize them in mitochondria. In this study, we aimed to explore a novel and direct role of mitochondrial fission in lipid homeostasis in HeLa cells. We observed the association between mitochondrial morphology and lipid droplet accumulation in response to high exogenous fatty acids. We inhibited mitochondrial fission by silencing dynamin-related protein 1(DRP1) and observed the shift in fatty acid storage-usage balance. Inhibition of mitochondrial fission resulted in an increase in fatty acid content of lipid droplets and a decrease in mitochondrial fatty acid oxidation. Next, we overexpressed carnitine palmitoyltransferase-1 (CPT1), a key mitochondrial protein in fatty acid oxidation, to further examine the relationship between mitochondrial fatty acid usage and mitochondrial morphology. Mitochondrial fission plays a role in distributing exogenous fatty acids. CPT1A controlled the respiratory rate of mitochondrial fatty acid oxidation but did not cause a shift in the distribution of fatty acids between mitochondria and lipid droplets. Our data reveals a novel function for mitochondrial fission in balancing exogenous fatty acids between usage and storage, assigning a role for mitochondrial dynamics in control of intracellular fuel utilization and partitioning. |
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| DOI: | doi:10.3390/metabo11050322 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.3390/metabo11050322 |
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| | Volltext: https://www.mdpi.com/2218-1989/11/5/322 |
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| | DOI: https://doi.org/10.3390/metabo11050322 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | fatty acid oxidation |
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| | lipid homeostasis |
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| | mitochondrial dynamics |
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| K10plus-PPN: | 1764715985 |
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| Verknüpfungen: | → Zeitschrift |
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Mitochondrial fission governed by Drp1 regulates exogenous fatty acid usage and storage in hela cells / Song, Jae-Eun [VerfasserIn]; 18 May 2021 (Online-Ressource)
