| |  Online-Ressource |
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| Verfasst von: | Fogeron, Marie-Laure [VerfasserIn]  |
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| | Paul, David [VerfasserIn] |
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| | Jirasko, Vlastimil [VerfasserIn] |
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| | Bartenschlager, Ralf [VerfasserIn] |
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| Titel: | Functional expression, purification, characterization, and membrane reconstitution of non-structural protein 2 from hepatitis C virus |
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| Verf.angabe: | Marie-Laure Fogeron, David Paul, Vlastimil Jirasko, Roland Montserret, Denis Lacabanne, Jennifer Molle, Aurélie Badillo, Célia Boukadida, Sonia Georgeault, Philippe Roingeard, Annette Martin, Ralf Bartenschlager, François Penin, Anja Böckmann |
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| E-Jahr: | 2015 |
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| Jahr: | 29 August 2015 |
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| Umfang: | 6 S. |
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| Teil: | volume:116 |
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| | year:2015 |
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| | pages:1-6 |
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| | extent:6 |
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| Fussnoten: | Gesehen am 03.08.2021 |
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| Titel Quelle: | Enthalten in: Protein expression and purification |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier, 1990 |
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| Jahr Quelle: | 2015 |
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| Band/Heft Quelle: | 116(2015), Seite 1-6 |
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| ISSN Quelle: | 1096-0279 |
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| Abstract: | Non-structural protein 2 (NS2) of the hepatitis C virus (HCV) is an integral membrane protein that contains a cysteine protease and that plays a central organizing role in assembly of infectious progeny virions. While the crystal structure of the protease domain has been solved, the NS2 full-length form remains biochemically and structurally uncharacterized because recombinant NS2 could not be prepared in sufficient quantities from cell-based systems. We show here that functional NS2 in the context of the NS2-NS3pro precursor protein, ensuring NS2-NS3 cleavage, can be efficiently expressed by using a wheat germ cell-free expression system. In this same system, we subsequently successfully produce and purify milligram amounts of a detergent-solubilized form of full-length NS2 exhibiting the expected secondary structure content. Furthermore, immuno-electron microscopy analyses of reconstituted proteoliposomes demonstrate NS2 association with model membranes. |
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| DOI: | doi:10.1016/j.pep.2015.08.027 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.pep.2015.08.027 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S1046592815300474 |
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| | DOI: https://doi.org/10.1016/j.pep.2015.08.027 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Cell-free protein expression |
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| | Hepatitis C virus |
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| | Lipid reconstitution |
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| | Membrane protein |
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| | Non-structural protein 2 |
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| K10plus-PPN: | 1765315433 |
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| Verknüpfungen: | → Zeitschrift |
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Functional expression, purification, characterization, and membrane reconstitution of non-structural protein 2 from hepatitis C virus / Fogeron, Marie-Laure [VerfasserIn]; 29 August 2015 (Online-Ressource)
