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Verfasst von:Probst, Katja [VerfasserIn]   i
 Nurjadi, Dennis [VerfasserIn]   i
 Heeg, Klaus [VerfasserIn]   i
 Frede, Anne-Marie [VerfasserIn]   i
 Dalpke, Alexander [VerfasserIn]   i
 Boutin, Sébastien [VerfasserIn]   i
Titel:Molecular detection of carbapenemases in enterobacterales
Titelzusatz:a comparison of real-time multiplex PCR and whole-genome sequencing
Verf.angabe:Katja Probst, Dennis Nurjadi, Klaus Heeg, Anne-Marie Frede, Alexander H. Dalpke and Sébastien Boutin
E-Jahr:2021
Jahr:16 June 2021
Umfang:8 S.
Fussnoten:Gesehen am 05.08.2021
Titel Quelle:Enthalten in: Antibiotics
Ort Quelle:Basel : MDPI, 2012
Jahr Quelle:2021
Band/Heft Quelle:10(2021), 6, Artikel-ID 726, Seite 1-8
ISSN Quelle:2079-6382
Abstract:Carbapenem-resistant Enterobacterales are a growing problem in healthcare systems worldwide. While whole-genome sequencing (WGS) has become a powerful tool for analyzing transmission and possible outbreaks, it remains laborious, and the limitations in diagnostic workflows are not well studied. The aim of this study was to compare the performance of WGS and real-time multiplex PCR (RT-qPCR) for diagnosing carbapenem-resistant Enterobacterales. In this study, we analyzed 92 phenotypically carbapenem-resistant Enterobacterales, sent to the University Hospital Heidelberg in 2019, by the carbapenem inactivation method (CIM) and compared WGS and RT-qPCR as genotypic carbapenemase detection methods. In total, 80.4% of the collected isolates were identified as carbapenemase producers. For six isolates, discordant results were recorded for WGS, PCR and CIM, as the carbapenemase genes were initially not detected by WGS. A reanalysis using raw reads, rather than assembly, highlighted a coverage issue with failure to detect carbapenemases located in contigs with a coverage lower than 10×, which were then discarded. Our study shows that multiplex RT-qPCR and CIM can be a simple alternative to WGS for basic surveillance of carbapenemase-producing Enterobacterales. Using WGS in clinical workflow has some limitations, especially regarding coverage and sensitivity. We demonstrate that antimicrobial resistance gene detection should be performed on the raw reads or non-curated draft genome to increase sensitivity.
DOI:doi:10.3390/antibiotics10060726
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.3390/antibiotics10060726
 Volltext: https://www.mdpi.com/2079-6382/10/6/726
 DOI: https://doi.org/10.3390/antibiotics10060726
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:antimicrobial resistance
 carbapenem inactivation method
 carbapenem-resistant Enterobacterales
 real-time multiplex PCR
 whole-genome sequencing
K10plus-PPN:1765673089
Verknüpfungen:→ Zeitschrift

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