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| Verfasst von: | Klupp, Fee [VerfasserIn]  |
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| | Kahlert, Christoph [VerfasserIn] |
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| | Franz, Clemens [VerfasserIn] |
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| | Halama, Niels [VerfasserIn] |
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| | Schleußner, Nikolai [VerfasserIn] |
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| | Wirsik, Naita M. [VerfasserIn] |
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| | Warth, Arne [VerfasserIn] |
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| | Schmidt, Thomas [VerfasserIn] |
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| | Ulrich, Alexis [VerfasserIn] |
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| Titel: | Granulin |
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| Titelzusatz: | an invasive and survival-determining marker in colorectal cancer patients |
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| Verf.angabe: | Fee Klupp, Christoph Kahlert, Clemens Franz, Niels Halama, Nikolai Schleussner, Naita M. Wirsik, Arne Warth, Thomas Schmidt and Alexis B. Ulrich |
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| E-Jahr: | 2021 |
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| Jahr: | 16 June 2021 |
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| Umfang: | 11 S. |
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| Fussnoten: | Gesehen am 05.08.2021 |
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| Titel Quelle: | Enthalten in: International journal of molecular sciences |
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| Ort Quelle: | Basel : Molecular Diversity Preservation International, 2000 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 22(2021), 12, Artikel-ID 6436, Seite 1-11 |
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| ISSN Quelle: | 1422-0067 |
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| | 1661-6596 |
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| Abstract: | Background: Granulin is a secreted, glycosylated peptide—originated by cleavage from a precursor protein—which is involved in cell growth, tumor invasion and angiogenesis. However, the specific prognostic impact of granulin in human colorectal cancer has only been studied to a limited extent. Thus, we wanted to assess the expression of granulin in colorectal cancer patients to evaluate its potential as a prognostic biomarker. Methods: Expressional differences of granulin in colorectal carcinoma tissue (n = 94) and corresponding healthy colon mucosa were assessed using qRT-PCR. Immunohistochemistry was performed in colorectal cancer specimens (n = 97), corresponding healthy mucosa (n = 47) and colorectal adenomas (n = 19). Subsequently, the results were correlated with histopathological and clinical patients’ data. HCT-116 cells were transfected with siRNA for invasion and migration assays. Results: Immunohistochemistry and qRT-PCR revealed tumoral over expression of granulin in colorectal cancer specimens compared to corresponding healthy colon mucosa and adenomas. Tumoral overexpression of granulin was associated with a significantly impaired overall survival. Moreover, downregulation of granulin by siRNA significantly diminished the invasive capacities of HCT-116 cells in vitro. Conclusion: Expression of granulin differs in colorectal cancer tissue, adenomas and healthy colon mucosa. Furthermore, granulin features invasive and migrative capabilities and overexpression of granulin correlates with a dismal prognosis. This reveals its potential as a prognostic biomarker and granulin could be a worthwhile molecular target for individualized anticancer therapy. |
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| DOI: | doi:10.3390/ijms22126436 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.3390/ijms22126436 |
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| | Volltext: https://www.mdpi.com/1422-0067/22/12/6436 |
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| | DOI: https://doi.org/10.3390/ijms22126436 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | adenomas |
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| | colorectal cancer |
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| | granulin |
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| | GRN |
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| | survival |
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| K10plus-PPN: | 1765678595 |
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| Verknüpfungen: | → Zeitschrift |
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