| |  Online-Ressource |
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| Verfasst von: | Cunha Navarro, Isabela [VerfasserIn]  |
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| | Tuorto, Francesca [VerfasserIn] |
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| | Jordan, David [VerfasserIn] |
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| | Legrand, Carine [VerfasserIn] |
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| | Price, Jonathan [VerfasserIn] |
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| | Braukmann, Fabian [VerfasserIn] |
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| | Hendrick, Alan G [VerfasserIn] |
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| | Akay, Alper [VerfasserIn] |
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| | Kotter, Annika [VerfasserIn] |
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| | Helm, Mark [VerfasserIn] |
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| | Lyko, Frank [VerfasserIn] |
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| | Miska, Eric [VerfasserIn] |
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| Titel: | Translational adaptation to heat stress is mediated by RNA 5-methylcytosine in Caenorhabditis elegans |
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| Verf.angabe: | Isabela Cunha Navarro, Francesca Tuorto, David Jordan, Carine Legrand, Jonathan Price, Fabian Braukmann, Alan G Hendrick, Alper Akay, Annika Kotter, Mark Helm, Frank Lyko & Eric A Miska |
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| Jahr: | 2021 |
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| Umfang: | 18 S. |
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| Fussnoten: | Veröffentlicht am 7. Dezember 2020 ; Gesehen am 10.08.2021 |
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| Titel Quelle: | Enthalten in: European Molecular Biology OrganizationThe EMBO journal |
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| Ort Quelle: | [London] : Nature Publishing Group UK, 1982 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 40(2021), 6, Artikel-ID e105496, Seite 1-18 |
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| ISSN Quelle: | 1460-2075 |
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| Abstract: | Abstract Methylation of carbon-5 of cytosines (m5C) is a post-transcriptional nucleotide modification of RNA found in all kingdoms of life. While individual m5C-methyltransferases have been studied, the impact of the global cytosine-5 methylome on development, homeostasis and stress remains unknown. Here, using Caenorhabditis elegans, we generated the first organism devoid of m5C in RNA, demonstrating that this modification is non-essential. Using this genetic tool, we determine the localisation and enzymatic specificity of m5C sites in the RNome in vivo. We find that NSUN-4 acts as a dual rRNA and tRNA methyltransferase in C. elegans mitochondria. In agreement with leucine and proline being the most frequently methylated tRNA isoacceptors, loss of m5C impacts the decoding of some triplets of these two amino acids, leading to reduced translation efficiency. Upon heat stress, m5C loss leads to ribosome stalling at UUG triplets, the only codon translated by an m5C34-modified tRNA. This leads to reduced translation efficiency of UUG-rich transcripts and impaired fertility, suggesting a role of m5C tRNA wobble methylation in the adaptation to higher temperatures. |
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| DOI: | doi:10.15252/embj.2020105496 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.15252/embj.2020105496 |
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| | Volltext: https://www.embopress.org/doi/full/10.15252/embj.2020105496 |
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| | DOI: https://doi.org/10.15252/embj.2020105496 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | 5-methylcytosine |
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| | Caenorhabditis elegans |
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| | NSUN |
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| | RNA modifications |
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| | translation efficiency |
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| K10plus-PPN: | 1765992672 |
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| Verknüpfungen: | → Zeitschrift |
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Translational adaptation to heat stress is mediated by RNA 5-methylcytosine in Caenorhabditis elegans / Cunha Navarro, Isabela [VerfasserIn]; 2021 (Online-Ressource)
