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Verfasst von:Georgiev, Tihomir [VerfasserIn]   i
 Svirin, Mikhail [VerfasserIn]   i
 Jaimovich, Enrique [VerfasserIn]   i
 Fink, Rainer [VerfasserIn]   i
Titel:Localized nuclear and perinuclear Ca2+ signals in intact mouse skeletal muscle fibers
Verf.angabe:Tihomir Georgiev, Mikhail Svirin, Enrique Jaimovich and Rainer H.A. Fink
E-Jahr:2015
Jahr:29 September 2015
Umfang:15 S.
Teil:year:2015
 number:6
 elocationid:263
 pages:1-15
 extent:15
Fussnoten:Im Titel sind die Zahl 2 und das Zeichen + hochgestellt ; Gesehen am 10.08.2021
Titel Quelle:Enthalten in: Frontiers in physiology
Ort Quelle:Lausanne : Frontiers Research Foundation, 2007
Jahr Quelle:2015
Band/Heft Quelle:(2015), 6, Artikel-ID 263, Seite 1-15
ISSN Quelle:1664-042X
Abstract:Nuclear Ca2+ is important for the regulation of several nuclear processes such as gene expression. Localized Ca2+ signals (LCSs) in skeletal muscle fibers of mice have been mainly studied as Ca2+ release events from the sarcoplasmic reticulum. Their location with regard to cell nuclei has not been investigated. Our study is based on the hypothesis that LCSs associated with nuclei are present in skeletal muscle fibers of adult mice. Therefore we carried out experiments addressing this question and we found novel Ca2+ signals associated with nuclei of skeletal muscle fibers (with possibly attached satellite cells). We measured localized nuclear and perinuclear Ca2+ signals (NLCSs and PLCSs) alongside cytosolic localized Ca2+ signals (CLCSs) during a hypertonic treatment. We also observed NLCSs under isotonic conditions. The NLCSs and PLCSs are Ca2+ signals in the range of micrometer (FWHM (full width at half maximum): 2.75 µm±0.27 µm (NLCSs) and 2.55 µm±0.17 µm (PLCSs), S.E.M.). Additionally, global nuclear Ca2+ signals (NGCSs) were observed. To investigate which type of Ca2+ channels contribute to the Ca2+ signals associated with nuclei in skeletal muscle fibers, we performed measurements with the RyR blocker dantrolene, the DHPR blocker nifedipine or the IP3R blocker Xestospongin C. We observed Ca2+ signals associated with nuclei in the presence of each blocker. Nifedipine and dantrolene had an inhibitory effect on the fraction of fibers with PLCSs. The situation for the fraction of fibers with NLCSs is more complex indicating that RyR is less important for the generation of NLCSs compared to the generation of PLCSs. The fraction of fibers with NLCSs and PLCSs is not reduced in the presence of Xestospongin C. The localized perinuclear and intranuclear Ca2+ signals may be a powerful tool for the cell to regulate adaptive processes as gene expression. The intranuclear Ca2+ signals may be particularly interesting in this respect.
DOI:doi:10.3389/fphys.2015.00263
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.3389/fphys.2015.00263
 Volltext: https://www.frontiersin.org/articles/10.3389/fphys.2015.00263/full
 DOI: https://doi.org/10.3389/fphys.2015.00263
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:global nuclear Ca2+ signals
 Hypertonic treatment
 localized nuclear Ca2+ signals
 localized perinuclear Ca2+ signals
 organelle Ca2+
 satellite cells
K10plus-PPN:176599392X
Verknüpfungen:→ Zeitschrift

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