Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Georgiev, Tihomir [VerfasserIn]   i
 Förderer, Moritz [VerfasserIn]   i
 Fink, Rainer [VerfasserIn]   i
 Vogel, Martin [VerfasserIn]   i
Titel:Colocalization properties of elementary Ca2+ release signals with structures specific to the contractile filaments and the tubular system of intact mouse skeletal muscle fibers
Verf.angabe:Tihomir Georgiev, Bolek Zapiec, Moritz Förderer, Rainer H.A. Fink, Martin Vogel
E-Jahr:2015
Jahr:21 October 2015
Umfang:10 S.
Fussnoten:Im Titel ist die Zahl 2 und das Zeichen + hochgestellt ; Gesehen am 10.08.2021
Titel Quelle:Enthalten in: Journal of structural biology
Ort Quelle:San Diego, Calif. : Elsevier, 1990
Jahr Quelle:2015
Band/Heft Quelle:192(2015), 3, Seite 366-375
ISSN Quelle:1095-8657
Abstract:Ca2+ regulates several important intracellular processes. We combined second harmonic generation (SHG) and two photon excited fluorescence microscopy (2PFM) to simultaneously record the SHG signal of the myosin filaments and localized elementary Ca2+ release signals (LCSs). We found LCSs associated with Y-shaped structures of the myosin filament pattern (YMs), so called verniers, in intact mouse skeletal muscle fibers under hypertonic treatment. Ion channels crucial for the Ca2+ regulation are located in the tubular system, a system that is important for Ca2+ regulation and excitation-contraction coupling. We investigated the tubular system of intact, living mouse skeletal muscle fibers using 2PFM and the fluorescent Ca2+ indicator Fluo-4 dissolved in the external solution or the membrane dye di-8-ANEPPS. We simultaneously measured the SHG signal from the myosin filaments of the skeletal muscle fibers. We found that at least a subset of the YMs observed in SHG images are closely juxtaposed with Y-shaped structures of the transverse tubules (YTs). The distances of corresponding YMs and YTs yield values between 1.3μm and 4.1μm including pixel uncertainty with a mean distance of 2.52±0.10μm (S.E.M., n=41). Additionally, we observed that some of the linear-shaped areas in the tubular system are colocalized with linear-shaped areas in the SHG images.
DOI:doi:10.1016/j.jsb.2015.09.018
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1016/j.jsb.2015.09.018
 Volltext: https://www.sciencedirect.com/science/article/pii/S1047847715300733
 DOI: https://doi.org/10.1016/j.jsb.2015.09.018
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Longitudinal tubules
 Second harmonic generation microscopy
 Verniers
 Z tubule
K10plus-PPN:1765995442
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68768455   QR-Code
zum Seitenanfang