| |  Online-Ressource |
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| Verfasst von: | Lau, Benjamin [VerfasserIn]  |
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| | Cheng, Jingdong [VerfasserIn] |
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| | Flemming, Dirk [VerfasserIn] |
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| | La Venuta, Giuseppe [VerfasserIn] |
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| | Berninghausen, Otto [VerfasserIn] |
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| | Beckmann, Roland [VerfasserIn] |
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| | Hurt, Ed [VerfasserIn] |
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| Titel: | Structure of the maturing 90S pre-ribosome in association with the RNA exosome |
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| Verf.angabe: | Benjamin Lau, Jingdong Cheng, Dirk Flemming, Giuseppe La Venuta, Otto Berninghausen, Roland Beckmann, Ed Hurt |
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| E-Jahr: | 2021 |
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| Jahr: | 21 January 2021 |
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| Umfang: | 15 S. |
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| Fussnoten: | Online veröffentlicht am 15. Dezember 2020, Artikelversion vom 21. Januar 2021 ; Gesehen am 10.08.2021 |
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| Titel Quelle: | Enthalten in: Molecular cell |
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| Ort Quelle: | [Cambridge, Mass.] : Cell Press, 1997 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 81(2021), 2 vom: Jan., Seite 293-303, e1-e4 |
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| ISSN Quelle: | 1097-4164 |
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| Abstract: | Ribosome assembly is catalyzed by numerous trans-acting factors and coupled with irreversible pre-rRNA processing, driving the pathway toward mature ribosomal subunits. One decisive step early in this progression is removal of the 5' external transcribed spacer (5'-ETS), an RNA extension at the 18S rRNA that is integrated into the huge 90S pre-ribosome structure. Upon endo-nucleolytic cleavage at an internal site, A(1), the 5'-ETS is separated from the 18S rRNA and degraded. Here we present biochemical and cryo-electron microscopy analyses that depict the RNA exosome, a major 3'-5' exoribonuclease complex, in a super-complex with the 90S pre-ribosome. The exosome is docked to the 90S through its co-factor Mtr4 helicase, a processive RNA duplex-dismantling helicase, which strategically positions the exosome at the base of 5'-ETS helices H9-H9', which are dislodged in our 90S-exosome structures. These findings suggest a direct role of the exosome in structural remodeling of the 90S pre-ribosome to drive eukaryotic ribosome synthesis. |
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| DOI: | doi:10.1016/j.molcel.2020.11.009 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.molcel.2020.11.009 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S1097276520307875 |
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| | DOI: https://doi.org/10.1016/j.molcel.2020.11.009 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | arch domain |
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| | complex |
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| | core |
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| | crystal-structure |
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| | endonuclease |
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| | mtr4 |
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| | proteins |
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| | ssu processome |
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| | subunit |
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| | visualization |
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| K10plus-PPN: | 1766008372 |
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| Verknüpfungen: | → Zeitschrift |
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Structure of the maturing 90S pre-ribosome in association with the RNA exosome / Lau, Benjamin [VerfasserIn]; 21 January 2021 (Online-Ressource)
