| |  Online-Ressource |
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| Verfasst von: | Zilova, Lucie [VerfasserIn]  |
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| | Weinhardt, Venera [VerfasserIn] |
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| | Tavhelidse-Suck, Tinatini [VerfasserIn] |
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| | Schlagheck, Christina [VerfasserIn] |
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| | Thumberger, Thomas [VerfasserIn] |
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| | Wittbrodt, Joachim [VerfasserIn] |
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| Titel: | Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development |
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| Verf.angabe: | Lucie Zilova, Venera Weinhardt, Tinatini Tavhelidse, Christina Schlagheck, Thomas Thumberger, Joachim Wittbrodt |
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| E-Jahr: | 2021 |
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| Jahr: | 12 July 2021 |
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| Umfang: | 26 S. |
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| Fussnoten: | Gesehen am 10.08.2021 |
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| Titel Quelle: | Enthalten in: eLife |
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| Ort Quelle: | Cambridge : eLife Sciences Publications, 2012 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 10(2021), Artikel-ID e66998, Seite 1-26 |
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| ISSN Quelle: | 2050-084X |
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| Abstract: | Organoids derived from pluripotent stem cells promise the solution to current challenges in basic and biomedical research. Mammalian organoids are however limited by long developmental time, variable success, and lack of direct comparison to an in vivo reference. To overcome these limitations and address species-specific cellular organization, we derived organoids from rapidly developing teleosts. We demonstrate how primary embryonic pluripotent cells from medaka and zebrafish efficiently assemble into anterior neural structures, particularly retina. Within 4 days, blastula-stage cell aggregates reproducibly execute key steps of eye development: retinal specification, morphogenesis, and differentiation. The number of aggregated cells and genetic factors crucially impacted upon the concomitant morphological changes that were intriguingly reflecting the in vivo situation. High efficiency and rapid development of fish-derived organoids in combination with advanced genome editing techniques immediately allow addressing aspects of development and disease, and systematic probing of impact of the physical environment on morphogenesis and differentiation. |
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| DOI: | doi:10.7554/eLife.66998 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext ; Verlag: https://doi.org/10.7554/eLife.66998 |
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| | kostenfrei: Volltext: https://elifesciences.org/articles/66998 |
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| | DOI: https://doi.org/10.7554/eLife.66998 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Bibliogr. Hinweis: | Forschungsdaten: Zilova, Lucie: Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development - in vivo imaging of OV formation |
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| Sach-SW: | anterior neuroectoderm |
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| | crucial role |
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| | homeobox gene |
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| | neural differentiation |
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| | optic-cup |
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| | pigment-epithelium |
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| | stem-cells |
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| | transcription factor |
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| | vertebrate eye |
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| | zebrafish |
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| K10plus-PPN: | 1766015395 |
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| Verknüpfungen: | → Zeitschrift |
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Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development / Zilova, Lucie [VerfasserIn]; 12 July 2021 (Online-Ressource)
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