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Verfasst von:Narayanasamy, Kaarjel K. [VerfasserIn]   i
 Stojic, Aleksandar [VerfasserIn]   i
 Li, Yunqing [VerfasserIn]   i
 Saß, Steffen [VerfasserIn]   i
 Hesse, Marina R. [VerfasserIn]   i
 Deussner-Helfmann, Nina S. [VerfasserIn]   i
 Dietz, Marina S. [VerfasserIn]   i
 Kuner, Thomas [VerfasserIn]   i
 Klevanski, Maja [VerfasserIn]   i
 Heilemann, Mike [VerfasserIn]   i
Titel:Visualizing synaptic multi-protein patterns of neuronal tissue with DNA-assisted single-molecule localization microscopy
Verf.angabe:Kaarjel K. Narayanasamy, Aleksandar Stojic, Yunqing Li, Steffen Sass, Marina R. Hesse, Nina S. Deussner-Helfmann, Marina S. Dietz, Thomas Kuner, Maja Klevanski and Mike Heilemann
E-Jahr:2021
Jahr:17 June 2021
Umfang:9 S.
Teil:volume:13
 year:2021
 elocationid:32
 pages:1-9
 extent:9
Fussnoten:Gesehen am 13.08.2021
Titel Quelle:Enthalten in: Frontiers in synaptic neuroscience
Ort Quelle:Lausanne : Frontiers Research Foundation, 2009
Jahr Quelle:2021
Band/Heft Quelle:13(2021), Artikel-ID 32, Seite 1-9
ISSN Quelle:1663-3563
Abstract:The development of super-resolution microscopy (SRM) has widened our understanding of biomolecular structure and function in biological materials. Imaging multiple targets within a single area would elucidate their spatial localization relative to the cell matrix and neighboring biomolecules, revealing multi-protein macromolecular structures and their functional co-dependencies. SRM methods are, however, limited to the number of suitable fluorophores that can be imaged during a single acquisition as well as the loss of antigens during antibody washing and restaining for organic dye multiplexing. We report the visualization of multiple protein targets within the pre- and postsynapse in 350-400 nm thick neuronal tissue sections using DNA-assisted single-molecule localization microscopy (SMLM). In a single labeling step, antibodies conjugated with short DNA oligonucleotides visualized multiple targets by sequential exchange of fluorophore-labeled complementary oligonucleotides present in the imaging buffer. This approach avoids potential effects on structural integrity when using multiple rounds of immunolabeling and eliminates chromatic aberration, because all targets are imaged using a single excitation laser wavelength. This method proved robust for multi-target imaging in semi-thin tissue sections with a lateral resolution better than 25 nm, paving the way toward structural cell biology with single-molecule SRM.
DOI:doi:10.3389/fnsyn.2021.671288
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.3389/fnsyn.2021.671288
 Volltext: https://www.frontiersin.org/article/10.3389/fnsyn.2021.671288
 DOI: https://doi.org/10.3389/fnsyn.2021.671288
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1766755321
Verknüpfungen:→ Zeitschrift

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