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| Verfasst von: | Kamionka, Eva-Maria [VerfasserIn]  |
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| | Qian, Baifeng [VerfasserIn] |
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| | Gross, Wolfgang [VerfasserIn] |
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| | Bergmann, Frank [VerfasserIn] |
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| | Hackert, Thilo [VerfasserIn] |
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| | Beretta, Carlo Antonio [VerfasserIn] |
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| | Dross, Nicolas [VerfasserIn] |
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| | Ryschich, Eduard [VerfasserIn] |
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| Titel: | Collagen organization does not influence T-cell distribution in stroma of human pancreatic cancer |
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| Verf.angabe: | Eva-Maria Kamionka, Baifeng Qian, Wolfgang Gross, Frank Bergmann, Thilo Hackert, Carlo A. Beretta, Nicolas Dross and Eduard Ryschich |
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| E-Jahr: | 2021 |
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| Jahr: | 21 July 2021 |
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| Umfang: | 24 S. |
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| Fussnoten: | Gesehen am 13.08.2021 |
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| Titel Quelle: | Enthalten in: Cancers |
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| Ort Quelle: | Basel : MDPI, 2009 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 13(2021), 15 vom: 21. Juli, Artikel-ID 3648, Seite 1-24 |
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| ISSN Quelle: | 2072-6694 |
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| Abstract: | The dominant intrastromal T-cell infiltration in pancreatic cancer is mainly caused by the contact guidance through the excessive desmoplastic reaction and could represent one of the obstacles to an effective immune response in this tumor type. This study analyzed the collagen organization in normal and malignant pancreatic tissues as well as its influence on T-cell distribution in pancreatic cancer. Human pancreatic tissue was analyzed using immunofluorescence staining and multiphoton and SHG microscopy supported by multistep image processing. The influence of collagen alignment on activated T-cells was studied using 3D matrices and time-lapse microscopy. It was found that the stroma of malignant and normal pancreatic tissues was characterized by complex individual organization. T-cells were heterogeneously distributed in pancreatic cancer and there was no relationship between T-cell distribution and collagen organization. There was a difference in the angular orientation of collagen alignment in the peritumoral and tumor-cell-distant stroma regions in the pancreatic ductal adenocarcinoma tissue, but there was no correlation in the T-cell densities between these regions. The grade of collagen alignment did not influence the directionality of T-cell migration in the 3D collagen matrix. It can be concluded that differences in collagen organization do not change the spatial orientation of T-cell migration or influence stromal T-cell distribution in human pancreatic cancer. The results of the present study do not support the rationale of remodeling of stroma collagen organization for improvement of T-cell-tumor cell contact in pancreatic ductal adenocarcinoma. |
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| DOI: | doi:10.3390/cancers13153648 |
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| URL: | kostenfrei: Volltext ; Verlag: https://doi.org/10.3390/cancers13153648 |
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| | kostenfrei: Volltext: https://www.mdpi.com/2072-6694/13/15/3648 |
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| | DOI: https://doi.org/10.3390/cancers13153648 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | chemokines |
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| | collagen organization |
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| | human pancreatic cancer |
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| | T-cell infiltration |
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| | tumor stroma |
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| K10plus-PPN: | 1766795676 |
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| Verknüpfungen: | → Zeitschrift |
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| Lokale URL UB: |  Zum Volltext |
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Collagen organization does not influence T-cell distribution in stroma of human pancreatic cancer / Kamionka, Eva-Maria [VerfasserIn]; 21 July 2021 (Online-Ressource)
68770579
