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| Verfasst von: | Franzen, Julia [VerfasserIn]  |
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| | Georgomanolis, Theodoros [VerfasserIn] |
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| | Selich, Anton [VerfasserIn] |
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| | Kuo, Chao-Chung [VerfasserIn] |
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| | Stöger, Reinhard [VerfasserIn] |
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| | Brant, Lilija [VerfasserIn] |
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| | Mulabdić, Melita Sara [VerfasserIn] |
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| | Fernandez-Rebollo, Eduardo [VerfasserIn] |
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| | Grezella, Clara [VerfasserIn] |
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| | Ostrowska, Alina [VerfasserIn] |
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| | Begemann, Matthias [VerfasserIn] |
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| | Nikolić, Miloš [VerfasserIn] |
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| | Rath, Björn [VerfasserIn] |
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| | Ho, Anthony Dick [VerfasserIn] |
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| | Rothe, Michael [VerfasserIn] |
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| | Schambach, Axel [VerfasserIn] |
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| | Papantonis, Argyris [VerfasserIn] |
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| | Wagner, Wolfgang [VerfasserIn] |
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| Titel: | DNA methylation changes during long-term in vitro cell culture are caused by epigenetic drift |
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| Verf.angabe: | Julia Franzen, Theodoros Georgomanolis, Anton Selich, Chao-Chung Kuo, Reinhard Stöger, Lilija Brant, Melita Sara Mulabdić, Eduardo Fernandez-Rebollo, Clara Grezella, Alina Ostrowska, Matthias Begemann, Miloš Nikolić, Björn Rath, Anthony D. Ho, Michael Rothe, Axel Schambach, Argyris Papantonis & Wolfgang Wagner |
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| E-Jahr: | 2021 |
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| Jahr: | 19 May 2021 |
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| Umfang: | 12 S. |
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| Teil: | volume:4 |
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| | year:2021 |
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| | day:19 |
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| | month:05 |
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| | elocationid:598 |
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| | pages:1-12 |
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| | extent:12 |
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| Fussnoten: | Gesehen am 16.08.2021 |
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| Titel Quelle: | Enthalten in: Communications biology |
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| Ort Quelle: | London : Springer Nature, 2018 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 4(2021) vom: 19. Mai, Artikel-ID 598, Seite 1-12 |
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| ISSN Quelle: | 2399-3642 |
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| Abstract: | Culture expansion of primary cells evokes highly reproducible DNA methylation (DNAm) changes. We have identified CG dinucleotides (CpGs) that become continuously hyper- or hypomethylated during long-term culture of mesenchymal stem cells (MSCs) and other cell types. Bisulfite barcoded amplicon sequencing (BBA-seq) demonstrated that DNAm patterns of neighboring CpGs become more complex without evidence of continuous pattern development and without association to oligoclonal subpopulations. Circularized chromatin conformation capture (4C) revealed reproducible changes in nuclear organization between early and late passages, while there was no enriched interaction with other genomic regions that also harbor culture-associated DNAm changes. Chromatin immunoprecipitation of CTCF did not show significant differences during long-term culture of MSCs, however culture-associated hypermethylation was enriched at CTCF binding sites and hypomethylated CpGs were devoid of CTCF. Taken together, our results support the notion that DNAm changes during culture-expansion are not directly regulated by a targeted mechanism but rather resemble epigenetic drift. |
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| DOI: | doi:10.1038/s42003-021-02116-y |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1038/s42003-021-02116-y |
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| | Volltext: https://www.nature.com/articles/s42003-021-02116-y |
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| | DOI: https://doi.org/10.1038/s42003-021-02116-y |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1767117787 |
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| Verknüpfungen: | → Zeitschrift |
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DNA methylation changes during long-term in vitro cell culture are caused by epigenetic drift / Franzen, Julia [VerfasserIn]; 19 May 2021 (Online-Ressource)
