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| Verfasst von: | Fakuade, Funsho E. [VerfasserIn]  |
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| | Steckmeister, Vanessa [VerfasserIn] |
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| | Seibertz, Fitzwilliam [VerfasserIn] |
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| | Gronwald, Judith [VerfasserIn] |
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| | Kestel, Stefanie [VerfasserIn] |
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| | Menzel, Julia [VerfasserIn] |
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| | Pronto, Julius Ryan D. [VerfasserIn] |
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| | Taha, Karim [VerfasserIn] |
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| | Haghighi, Fereshteh [VerfasserIn] |
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| | Kensah, George [VerfasserIn] |
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| | Pearman, Charles M. [VerfasserIn] |
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| | Wiedmann, Felix [VerfasserIn] |
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| | Teske, Arco J. [VerfasserIn] |
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| | Schmidt, Constanze [VerfasserIn] |
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| | Dibb, Katharine M. [VerfasserIn] |
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| | El-Essawi, Aschraf [VerfasserIn] |
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| | Danner, Bernhard C. [VerfasserIn] |
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| | Baraki, Hassina [VerfasserIn] |
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| | Schwappach, Blanche [VerfasserIn] |
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| | Kutschka, Ingo [VerfasserIn] |
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| | Mason, Fleur E. [VerfasserIn] |
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| | Voigt, Niels [VerfasserIn] |
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| Titel: | Altered atrial cytosolic calcium handling contributes to the development of postoperative atrial fibrillation |
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| Verf.angabe: | Funsho E. Fakuade, Vanessa Steckmeister, Fitzwilliam Seibertz, Judith Gronwald, Stefanie Kestel, Julia Menzel, Julius Ryan D. Pronto, Karim Taha, Fereshteh Haghighi, George Kensah, Charles M. Pearman, Felix Wiedmann, Arco J. Teske, Constanze Schmidt, Katharine M. Dibb, Aschraf El-Essawi, Bernhard C. Danner, Hassina Baraki, Blanche Schwappach, Ingo Kutschka, Fleur E. Mason, and Niels Voigt |
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| E-Jahr: | 2021 |
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| Jahr: | 15 June 2021 |
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| Umfang: | 12 S. |
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| Teil: | volume:117 |
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| | year:2021 |
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| | number:7 |
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| | pages:1790-1801 |
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| | extent:12 |
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| Fussnoten: | Gesehen am 25.08.2021 |
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| Titel Quelle: | Enthalten in: Cardiovascular research |
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| Ort Quelle: | Oxford : Oxford University Press, 1967 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 117(2021), 7, Seite 1790-1801 |
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| ISSN Quelle: | 1755-3245 |
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| Abstract: | AIMS: Atrial fibrillation (AF) is a commonly occurring arrhythmia after cardiac surgery (postoperative AF, poAF) and is associated with poorer outcomes. Considering that reduced atrial contractile function is a predictor of poAF and that Ca2+ plays an important role in both excitation-contraction coupling and atrial arrhythmogenesis, this study aims to test whether alterations of intracellular Ca2+ handling contribute to impaired atrial contractility and to the arrhythmogenic substrate predisposing patients to poAF. - METHODS AND RESULTS: Right atrial appendages were obtained from patients in sinus rhythm undergoing open-heart surgery. Cardiomyocytes were investigated by simultaneous measurement of [Ca2+]i and action potentials (APs, patch-clamp). Patients were followed-up for 6 days to identify those with and without poAF. Speckle-tracking analysis of preoperative echocardiography revealed reduced left atrial contraction strain in poAF patients. At the time of surgery, cellular Ca2+ transients (CaTs) and the sarcoplasmic reticulum (SR) Ca2+ content were smaller in the poAF group. CaT decay was slower in poAF, but the decay of caffeine-induced Ca2+ transients was unaltered, suggesting preserved sodium-calcium exchanger function. In agreement, western blots revealed reduced SERCA2a expression in poAF patients but unaltered phospholamban expression/phosphorylation. Computational modelling indicated that reduced SERCA activity promotes occurrence of CaT and AP alternans. Indeed, alternans of CaT and AP occurred more often and at lower stimulation frequencies in atrial myocytes from poAF patients. Resting membrane potential and AP duration were comparable between both groups at various pacing frequencies (0.25-8 Hz). - CONCLUSIONS: Biochemical, functional, and modelling data implicate reduced SERCA-mediated Ca2+ reuptake into the SR as a major contributor to impaired preoperative atrial contractile function and to the pre-existing arrhythmogenic substrate in patients developing poAF. |
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| DOI: | doi:10.1093/cvr/cvaa162 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
DOI: https://doi.org/10.1093/cvr/cvaa162 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Action potential |
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| | Alternans |
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| | Arrhythmia |
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| | Calcium |
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| | Postoperative atrial fibrillation |
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| K10plus-PPN: | 1767870892 |
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| Verknüpfungen: | → Zeitschrift |
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Altered atrial cytosolic calcium handling contributes to the development of postoperative atrial fibrillation / Fakuade, Funsho E. [VerfasserIn]; 15 June 2021 (Online-Ressource)
