| |  Online-Ressource |
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| Verfasst von: | Wu, Silin [VerfasserIn]  |
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| | Greiner, Steffen [VerfasserIn] |
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| | Ma, Chongjian [VerfasserIn] |
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| | Zhong, Jiaxin [VerfasserIn] |
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| | Huang, Xiaojia [VerfasserIn] |
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| | Rausch, Thomas [VerfasserIn] |
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| | Zhao, Hongbo [VerfasserIn] |
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| Titel: | A fructan exohydrolase from maize degrades both inulin and levan and co-exists with 1-kestotriose in maize |
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| Verf.angabe: | Silin Wu, Steffen Greiner, Chongjian Ma, Jiaxin Zhong, Xiaojia Huang, Thomas Rausch and Hongbo Zhao |
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| E-Jahr: | 2021 |
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| Jahr: | 13 May 2021 |
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| Umfang: | 14 S. |
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| Teil: | volume:22 |
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| | year:2021 |
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| | number:10 |
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| | day:13 |
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| | month:05 |
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| | elocationid:5149 |
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| | pages:1-14 |
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| | extent:14 |
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| Fussnoten: | Gesehen am 06.09.2021 |
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| Titel Quelle: | Enthalten in: International journal of molecular sciences |
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| Ort Quelle: | Basel : Molecular Diversity Preservation International, 2000 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 22(2021), 10 vom: 13. Mai, Artikel-ID 5149, Seite 1-14 |
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| ISSN Quelle: | 1422-0067 |
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| | 1661-6596 |
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| Abstract: | Enzymes with fructan exohydrolase (FEH) activity are present not only in fructan-synthesizing species but also in non-fructan plants. This has led to speculation about their functions in non-fructan species. Here, a cell wall invertase-related Zm-6&1-FEH2 with no “classical” invertase motif was identified in maize. Following heterologous expression in Pichia pastoris and in Nicotiana benthamiana leaves, the enzyme activity of recombinant Zm-6&1-FEH2 displays substrate specificity with respect to inulin and levan. Subcellular localization showed Zm-6&1-FEH2 exclusively localized in the apoplast, and its expression profile was strongly dependent on plant development and in response to drought and abscisic acid. Furthermore, formation of 1-kestotriose, an oligofructan, was detected in vivo and in vitro and could be hydrolyzed by Zm-6&1-FEH2. In summary, these results support that Zm-6&1-FEH2 enzyme from maize can degrade both inulin-type and levan-type fructans, and the implications of the co-existence of Zm-6&1-FEH2 and 1-kestotriose are discussed. |
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| DOI: | doi:10.3390/ijms22105149 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.3390/ijms22105149 |
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| | Volltext: https://www.mdpi.com/1422-0067/22/10/5149 |
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| | DOI: https://doi.org/10.3390/ijms22105149 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | <i>Zea mays</i> |
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| | 1-kestotriose |
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| | fructan exohydrolase |
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| | inulin |
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| | levan |
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| K10plus-PPN: | 1769463925 |
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| Verknüpfungen: | → Zeitschrift |
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¬A¬ fructan exohydrolase from maize degrades both inulin and levan and co-exists with 1-kestotriose in maize / Wu, Silin [VerfasserIn]; 13 May 2021 (Online-Ressource)
