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| Verfasst von: | Laffert, Maximilian von [VerfasserIn]  |
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| | Warth, Arne [VerfasserIn] |
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| | Penzel, Roland [VerfasserIn] |
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| | Schirmacher, Peter [VerfasserIn] |
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| | Jonigk, Danny [VerfasserIn] |
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| | Kreipe, Hans [VerfasserIn] |
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| | Schildhaus, Hans-Ulrich [VerfasserIn] |
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| | Merkelbach-Bruse, Sabine [VerfasserIn] |
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| | Büttner, Reinhard [VerfasserIn] |
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| | Reu, Simone [VerfasserIn] |
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| | Kerler, Rosi [VerfasserIn] |
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| | Jung, Andreas [VerfasserIn] |
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| | Kirchner, Thomas [VerfasserIn] |
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| | Wölfel, Cornelius [VerfasserIn] |
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| | Petersen, Iver [VerfasserIn] |
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| | Rodriguez, Regulo [VerfasserIn] |
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| | Jochum, Wolfram [VerfasserIn] |
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| | Bartsch, Holger [VerfasserIn] |
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| | Fisseler-Eckhoff, Annette [VerfasserIn] |
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| | Berg, Erika [VerfasserIn] |
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| | Lenze, Dido [VerfasserIn] |
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| | Dietel, Manfred [VerfasserIn] |
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| | Hummel, Michael [VerfasserIn] |
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| Titel: | Anaplastic lymphoma kinase (ALK) gene rearrangement in non-small cell lung cancer (NSCLC) |
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| Titelzusatz: | results of a multi-centre ALK-testing |
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| Verf.angabe: | Maximilian v. Laffert, Arne Warth, Roland Penzel, Peter Schirmacher, Danny Jonigk, Hans Kreipe, Hans-Ulrich Schildhaus, Sabine Merkelbach-Bruse, Reinhard Büttner, Simone Reu, Rosi Kerler, Andreas Jung, Thomas Kirchner, Cornelius Wölfel, Iver Petersen, Regulo Rodriguez, Wolfram Jochum, Holger Bartsch, Annette Fisseler-Eckhoff, Erika Berg, Dido Lenze, Manfred Dietel, Michael Hummel |
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| E-Jahr: | 2013 |
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| Jahr: | 10 May 2013 |
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| Umfang: | 7 S. |
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| Fussnoten: | Gesehen am 21.09.2021 |
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| Titel Quelle: | Enthalten in: Lung cancer |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier, 1985 |
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| Jahr Quelle: | 2013 |
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| Band/Heft Quelle: | 81(2013), 2 vom: Aug., Seite 200-206 |
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| ISSN Quelle: | 1872-8332 |
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| Abstract: | Background - The reliable identification of non-small cell lung cancers (NSCLC) with chromosomal breaks in the gene of the anaplastic lymphoma kinase (ALK) is crucial for the induction of therapy with ALK-inhibitors. In order to ensure a reliable detection of ALK-breaks by means of fluorescence in situ hybridization (FISH) testing, round robin tests are essential. In preparation of a nation (German)-wide round robin test we initiated a pre-testing phase involving 8 experts in FISH-diagnostics to identify NSCLC cases (n=10) with a pre-tested ALK-status. In addition, ALK immunohistochemistry (IHC) was performed to assess ALK protein expression. - Material and methods - Sections derived from a tissue microarray, each consisting of 3 cores from 10 NSCLC cases, were independently tested for ALK protein expression by IHC and genomic ALK-breaks by FISH involving 8 institutes of pathology. Based on a pre-screening, 5 cases were identified to be clearly ALK-break negative, whereas the remaining 5 cases were ALK-break positive including one case with low percentage (20%) of positive cells. The latter had been additionally tested by RT-PCR. - Results - The 5 unequivocal ALK-break negative NSCLC were almost consistently scored negative by means of FISH and IHC by all 8 experts. Interestingly, 4 of the 5 cases with pre-defined ALK-breaks revealed homogenous FISH results whereas IHC for the detection of ALK protein expression showed heterogeneous results. The remaining case (low number of ALK-break positive cells) was scored negative by 3 experts and positive by the other 5. RT-PCR revealed the expression of an EML4-ALK fusion gene variant 1. - Conclusion - ALK-break negative NSCLC cases revealed concordant homogeneous results by means of FISH and IHC (score 0-1) by all 8 experts. Discordant FISH results were raised in one ALK-break positive case with a low number of affected tumor cells. The remaining 4 ALK-break positive cases revealed concordant FISH data whereas the ALK-IHC revealed very diverse results. The cases with concordant FISH results provide an excellent basis for round robin ALK-FISH testing. As long as standardized ALK-IHC protocols are missing, ALK protein expression cannot by regarded as the method of choice for identification of patients eligible for treatment with ALK inhibitors. |
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| DOI: | doi:10.1016/j.lungcan.2013.04.015 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.lungcan.2013.04.015 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S0169500213001657 |
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| | DOI: https://doi.org/10.1016/j.lungcan.2013.04.015 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Anaplastic lymphoma kinase (ALK) gene rearrangement |
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| | Fluorescence in situ hybridization (FISH) |
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| | Immunohistochemistry (IHC) |
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| | Non-small cell lung cancer (NSCLC) |
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| | Round robin test |
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| K10plus-PPN: | 1771289201 |
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| Verknüpfungen: | → Zeitschrift |
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Anaplastic lymphoma kinase (ALK) gene rearrangement in non-small cell lung cancer (NSCLC) / Laffert, Maximilian von [VerfasserIn]; 10 May 2013 (Online-Ressource)
