| |  Online-Ressource |
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| Verfasst von: | Erben, Esteban D. [VerfasserIn]  |
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| | Leiss, Kevin [VerfasserIn] |
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| | Liu, Bin [VerfasserIn] |
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| | Inchaustegui Gil, Diana Patricia [VerfasserIn] |
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| | Helbig, Claudia [VerfasserIn] |
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| | Clayton, Christine [VerfasserIn] |
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| Titel: | Insights into the functions and RNA binding of Trypanosoma brucei ZC3H22, RBP9 and DRBD7 |
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| Verf.angabe: | Esteban Erben, Kevin Leiss, Bin Liu, Diana Inchaustegui Gil, Claudia Helbig and Christine Clayton |
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| E-Jahr: | 2021 |
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| Jahr: | 04 February 2021 |
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| Umfang: | 10 S. |
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| Teil: | volume:148 |
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| | year:2021 |
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| | number:10 |
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| | month:09 |
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| | supplement:special issue 10 |
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| | pages:1186-1195 |
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| | extent:10 |
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| Fussnoten: | Gesehen am 22.09.2021 |
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| Titel Quelle: | Enthalten in: Parasitology |
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| Ort Quelle: | Cambridge [u.a.] : Cambridge Univ. Press, 1908 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 148(2021), 10, special issue 10 vom: Sept., Seite 1186-1195 |
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| ISSN Quelle: | 1469-8161 |
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| Abstract: | Trypanosoma brucei is unusually reliant on mRNA-binding proteins to control mRNA fate, because its protein-coding genes lack individual promoters. We here focus on three trypanosome RNA-binding proteins. ZC3H22 is specific to Tsetse fly forms, RBP9 is preferentially expressed in bloodstream forms; and DRBD7 is constitutively expressed. Depletion of RBP9 or DRBD7 did not affect bloodstream-form trypanosome growth. ZC3H22 depletion from procyclic forms caused cell clumping, decreased expression of genes required for cell growth and proliferation, and increased expression of some epimastigote markers. Apart from decreases in mRNAs encoding enzymes of glucose metabolism, levels of most ZC3H22-bound transcripts were unaffected by ZC3H22 depletion. We compared ZC3H22, RBP9 and DRBD7 RNA binding with that of 16 other RNA-binding proteins. ZC3H22, PUF3 and ERBP1 show a preference for ribosomal protein mRNAs. RBP9 preferentially binds mRNAs that are more abundant in bloodstream forms than in procyclic forms. RBP9, ZC3H5, ZC3H30 and DRBD7 prefer mRNAs with long coding regions; UBP1-associated mRNAs have long 3′-untranslated regions; and RRM1 prefers mRNAs with long 3′or 5′-untranslated regions. We suggest that proteins that prefer long mRNAs may have relatively short or degenerate binding sites, and that preferences for A or U increase binding in untranslated regions. |
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| DOI: | doi:10.1017/S0031182021000123 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1017/S0031182021000123 |
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| | Volltext: https://www.cambridge.org/core/journals/parasitology/article/insights-into-the-functions-and-rna-binding-of-trypanosoma- ... |
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| | DOI: https://doi.org/10.1017/S0031182021000123 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | mRNA |
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| | RNA-binding protein |
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| | Trypanosoma brucei |
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| K10plus-PPN: | 1771626542 |
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| Verknüpfungen: | → Zeitschrift |
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Insights into the functions and RNA binding of Trypanosoma brucei ZC3H22, RBP9 and DRBD7 / Erben, Esteban D. [VerfasserIn]; 04 February 2021 (Online-Ressource)
