Sex-specific differences in the development of acute alcohol-induced liver steatosis in mice

• Titel: Sex-specific differences in the development of acute alcohol-induced liver steatosis in mice
• Mitwirkende: Wagnerberger, Sabine
• Mitwirkende: Fiederlein, Lena
• Mitwirkende: Stahl, Carolin
• Mitwirkende: Millonig, Gunda
• Mitwirkende: Mueller, Sebastian
• Mitwirkende: Bischoff, Stephan C.
• Verf.angabe: Sabine Wagnerberger, Lena Fiederlein, Giridhar Kanuri, Carolin Stahl, Gunda Millonig, Sebastian Mueller, Stephan C. Bischoff and Ina Bergheim
• E-Jahr: 2013
• Jahr: 21 August 2013
• Umfang: Online-Ressource S.
• Umfang: 9 S.
• Illustrationen: Ill., graph. Darst.
• Teil: volume:48
• Teil: year:2013
• Teil: number:6
• Teil: pages:648-656
• Teil: extent:9
• Fussnoten: Gesehen am 23.09.2021
• Titel Quelle: In: Alcohol and alcoholism
• Ort Quelle: Oxford : Oxford Univ. Press, 1963
• Jahr Quelle: 2013
• Band/Heft Quelle: 48(2013), 6, Seite 648-656
• ISSN Quelle: 1464-3502
• DOI: doi:10.1093/alcalc/agt138
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 146663975X

Abstract

Aims: Results of several animal studies suggest that similar to humans, female rodents are more susceptible to chronic alcohol-induced liver disease (ALD). The aim of the present study was to determine whether female mice are more susceptible to acute alcohol-induced steatosis than male mice and to investigate possible mechanisms involved. Methods: Male and female C57BL/6J mice received one single dose of ethanol (6 g/kg bodyweight) or isocaloric maltose-dextrin solution intragastrically. Plasma alcohol concentration, markers of hepatic steatosis, activation of the TLR-4 signaling cascade and triglyceride export as well as lipid peroxidation and of iron metabolism were measured 12 h after acute alcohol intake. Results: In male and female ethanol-treated mice, plasma alcohol concentrations were still markedly increased 12 h after the alcohol challenge, which was associated with a significant accumulation of lipids in the liver and increase of transaminases in plasma; however, lipid accumulation was ∼3-fold higher in females in comparison with male animals. Expression of MyD88 was only found to be significantly induced in livers of female alcohol-exposed mice, whereas protein levels of ApoB were found to be significantly lower only in livers of female mice exposed to ethanol. Levels of 4-HNE protein adducts and ferritin were induced in livers of male and female ethanol-treated mice. Conclusion: Taken together, these data suggest that female mice are also more susceptible to acute alcohol-induced liver steatosis and that this involves an increased activation of TLR-4-dependent signaling pathways in the liver.