| Online-Ressource |
Verfasst von: | Seeßle, Jessica [VerfasserIn]  |
| Wenz, Theresa [VerfasserIn]  |
| Schnitzler, Paul [VerfasserIn]  |
| Gsenger, Julia [VerfasserIn]  |
| Giese, Thomas [VerfasserIn]  |
| Merle, Uta [VerfasserIn]  |
Titel: | High rate of HSV-1 reactivation in invasively ventilated COVID-19 patients |
Titelzusatz: | immunological findings |
Verf.angabe: | Jessica Seeßle, Theresa Hippchen, Paul Schnitzler, Julia Gsenger, Thomas Giese, Uta Merle |
E-Jahr: | 2021 |
Jahr: | July 1, 2021 |
Umfang: | 13 S. |
Fussnoten: | Gesehen am 23.09.2021 |
Titel Quelle: | Enthalten in: PLOS ONE |
Ort Quelle: | San Francisco, California, US : PLOS, 2006 |
Jahr Quelle: | 2021 |
Band/Heft Quelle: | 16(2021), 7, Artikel-ID e0254129, Seite 1-13 |
ISSN Quelle: | 1932-6203 |
Abstract: | SARS-CoV-2 infection can lead to severe acute respiratory distress syndrome with the need of invasive ventilation. Pulmonary herpes simplex-1 (HSV-1) reactivation in invasively ventilated patients is a known phenomenon. To date very little is known about the frequency and the predisposing factors of HSV-1 reactivation in COVID-19. Therefore, we evaluated our cohort of invasively ventilated COVID-19 patients with severe pneumonia for HSV-1 in respiratory specimens and combined these results with functional immunomonitoring of the peripheral blood. Tracheal secretions and bronchial lavages were screened by PCR for HSV-1 positivity. Comprehensive immunophenotyping and quantitative gene expression analysis of Interferon-stimulated genes (IFI44L, MX1, RSAD2, ISIG15 and IFIT1) and IL-1 beta were performed in whole blood. Time course of infection beginning at symptom onset was grouped into three phases (“early” phase 1: day 1-10, “middle” phase 2: day 11-30 and “late” phase 3: day 31-40). Pulmonary HSV-1 reactivation was exclusively observed in the later phases 2 and 3 in 15 of 18 analyzed patients. By FACS analysis a significant increase in activated CD8 T cells (CD38+HLADR+) in phase 2 was found when compared with phase 1 (p<0.05). Expression of Interferon-stimulated genes (IFI44L, RSAD2 ISIG15, MX1, IFIT1) was significantly lower after HSV-1 detection than before. Taken together, reactivation of HSV-1 in the later phase of SARS-CoV-2- infection occurs in parallel with a drop of antiviral innate responsiveness as shown by decreased expression of Interferon-stimulated genes and a concurrent increase of highly activated CD38+HLADR+ CD8 T cells. |
DOI: | doi:10.1371/journal.pone.0254129 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1371/journal.pone.0254129 |
| Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0254129 |
| DOI: https://doi.org/10.1371/journal.pone.0254129 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | Blood |
| COVID 19 |
| Cytotoxic T cells |
| Genetic interference |
| Interferons |
| Respiratory infections |
| SARS CoV 2 |
| Secretion |
K10plus-PPN: | 1771742046 |
Verknüpfungen: | → Zeitschrift |
High rate of HSV-1 reactivation in invasively ventilated COVID-19 patients / Seeßle, Jessica [VerfasserIn]; July 1, 2021 (Online-Ressource)