| |  Online-Ressource |
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| Verfasst von: | Korkmaz-İçöz, Sevil [VerfasserIn]  |
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| | Kocer, Cenk [VerfasserIn] |
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| | Sayour, Alex Ali [VerfasserIn] |
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| | Kraft, Patricia [VerfasserIn] |
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| | Benker, Mona Isabella [VerfasserIn] |
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| | Abulizi, Sophia [VerfasserIn] |
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| | Georgevici, Adrian-Iustin [VerfasserIn] |
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| | Brlecic, Paige [VerfasserIn] |
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| | Radovits, Tamás [VerfasserIn] |
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| | Loganathan, Sivakkanan [VerfasserIn] |
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| | Karck, Matthias [VerfasserIn] |
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| | Szabó, Gábor [VerfasserIn] |
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| Titel: | The sodium-glucose cotransporter-2 inhibitor canagliflozin alleviates endothelial dysfunction following in vitro vascular ischemia/reperfusion injury in rats |
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| Verf.angabe: | Sevil Korkmaz-Icöz, Cenk Kocer, Alex A. Sayour, Patricia Kraft, Mona I. Benker, Sophia Abulizi, Adrian-Iustin Georgevici, Paige Brlecic, Tamás Radovits, Sivakkanan Loganathan, Matthias Karck and Gábor Szabó |
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| E-Jahr: | 2021 |
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| Jahr: | 21 July 2021 |
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| Umfang: | 21 S. |
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| Fussnoten: | Gesehen am 25.09.2021 |
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| Titel Quelle: | Enthalten in: International journal of molecular sciences |
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| Ort Quelle: | Basel : Molecular Diversity Preservation International, 2000 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 22(2021), 15, Artikel-ID 7774, Seite 1-21 |
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| ISSN Quelle: | 1422-0067 |
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| | 1661-6596 |
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| Abstract: | Vascular ischemia/reperfusion injury (IRI) contributes to graft failure and adverse clinical outcomes following coronary artery bypass grafting. Sodium-glucose-cotransporter (SGLT)-2-inhibitors have been shown to protect against myocardial IRI, irrespective of diabetes. We hypothesized that adding canagliflozin (CANA) (an SGLT-2-inhibitor) to saline protects vascular grafts from IRI. Aortic rings from non-diabetic rats were isolated and immediately mounted in organ bath chambers (control, n = 9-10 rats) or underwent cold ischemic preservation in saline, supplemented either with a DMSO vehicle (IR, n = 8-10 rats) or 50µM CANA (IR + CANA, n = 9-11 rats). Vascular function was measured, the expression of 88 genes using PCR-array was analyzed, and feature selection using machine learning was applied. Impaired maximal vasorelaxation to acetylcholine in the IR-group compared to controls was significantly ameliorated by CANA (IR 31.7 ± 3.2% vs. IR + CANA 51.9 ± 2.5%, p < 0.05). IR altered the expression of 17 genes. Ccl2, Ccl3, Ccl4, CxCr4, Fos, Icam1, Il10, Il1a and Il1b have been found to have the highest interaction. Compared to controls, IR significantly upregulated the mRNA expressions of Il1a and Il6, which were reduced by 1.5- and 1.75-fold with CANA, respectively. CANA significantly prevented the upregulation of Cd40, downregulated NoxO1 gene expression, decreased ICAM-1 and nitrotyrosine, and increased PECAM-1 immunoreactivity. CANA alleviates endothelial dysfunction following IRI. |
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| DOI: | doi:10.3390/ijms22157774 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.3390/ijms22157774 |
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| | Volltext: https://www.mdpi.com/1422-0067/22/15/7774 |
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| | DOI: https://doi.org/10.3390/ijms22157774 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | canagliflozin |
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| | diabetes mellitus |
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| | endothelial function |
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| | ischemia/reperfusion |
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| | sodium-glucose cotransporter-2 |
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| K10plus-PPN: | 177180226X |
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| Verknüpfungen: | → Zeitschrift |
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¬The¬ sodium-glucose cotransporter-2 inhibitor canagliflozin alleviates endothelial dysfunction following in vitro vascular ischemia/reperfusion injury in rats / Korkmaz-İçöz, Sevil [VerfasserIn]; 21 July 2021 (Online-Ressource)
