| |  Online-Ressource |
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| Verfasst von: | Zhang, Wei [VerfasserIn]  |
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| | Freichel, Marc [VerfasserIn] |
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| | Hoeven, Frank van der [VerfasserIn] |
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| | Nawroth, Peter Paul [VerfasserIn] |
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| | Katus, Hugo [VerfasserIn] |
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| | Kälble, Florian [VerfasserIn] |
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| | Zitron, Edgar [VerfasserIn] |
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| | Schwenger, Vedat [VerfasserIn] |
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| Titel: | Novel endothelial cell-specific AQP1 knockout mice confirm the crucial role of endothelial AQP1 in ultrafiltration during peritoneal dialysis |
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| Verf.angabe: | Wei Zhang, Marc Freichel, Frank van der Hoeven, Peter Paul Nawroth, Hugo Katus, Florian Kälble, Edgar Zitron, Vedat Schwenger |
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| E-Jahr: | 2016 |
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| Jahr: | January 13, 2016 |
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| Umfang: | 15 S. |
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| Teil: | volume:11 |
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| | year:2016 |
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| | number:1 |
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| | elocationid:e0145513 |
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| | pages:1-15 |
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| | extent:15 |
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| Fussnoten: | Gesehen am 04.10.2021 |
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| Titel Quelle: | Enthalten in: PLOS ONE |
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| Ort Quelle: | San Francisco, California, US : PLOS, 2006 |
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| Jahr Quelle: | 2016 |
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| Band/Heft Quelle: | 11(2016), 1, Artikel-ID e0145513, Seite 1-15 |
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| ISSN Quelle: | 1932-6203 |
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| Abstract: | The water channel aquaporin-1 (AQP1) mediates about 50% ultrafiltration during a 2-hour hypertonic dwell in global AQP1 knockout (AQP1-/-) mice. Although AQP1 is widely expressed in various cell types including mesothelial cells, the ultrafiltration has been assumed to be mediated via endothelial AQP1 of the peritoneum. The partial embryonic lethality and reduced body weight in AQP1-/- mice may reflect potential confounding phenotypic effects evoked by ubiquitous AQP1 deletion, which may interfere with functional analysis of endothelial AQP1. Using a Cre/loxP approach, we generated and characterised endothelial cell- and time-specific AQP1 knockout (AQP1fl/fl; Cdh5-Cre+) mice. Compared to controls, AQP1fl/fl; Cdh5-Cre+ mice showed no difference in an initial clinical and biological analysis at baseline, including body weight and survival. During a 1-hour 3.86% mini-peritoneal equilibration test (mini-PET), AQP1fl/fl; Cdh5-Cre+ mice exhibited strongly decreased indices for AQP1-related transcellular water transport (43.0% in net ultrafiltration, 93.0% in sodium sieving and 57.9% in free water transport) compared to controls. The transport rates for small solutes of urea and glucose were not significantly altered. Our data provide the first direct experimental evidence for the functional relevance of endothelial AQP1 to the fluid transport in peritoneal dialysis and thereby further validate essential predictions of the three-pore model of peritoneal transport. |
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| DOI: | doi:10.1371/journal.pone.0145513 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1371/journal.pone.0145513 |
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| | Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0145513 |
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| | DOI: https://doi.org/10.1371/journal.pone.0145513 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Endothelial cells |
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| | Genetically modified animals |
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| | Glucose |
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| | Mouse models |
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| | Polymerase chain reaction |
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| | Solute transport |
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| | Ultrafiltration |
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| | Urea |
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| K10plus-PPN: | 177228937X |
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| Verknüpfungen: | → Zeitschrift |
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Novel endothelial cell-specific AQP1 knockout mice confirm the crucial role of endothelial AQP1 in ultrafiltration during peritoneal dialysis / Zhang, Wei [VerfasserIn]; January 13, 2016 (Online-Ressource)
