| |  Online-Ressource |
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| Verfasst von: | Powers, Kyle T. [VerfasserIn]  |
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| | Stevenson-Jones, Flint [VerfasserIn] |
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| | Yadav, Sathish K N [VerfasserIn] |
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| | Amthor, Beate [VerfasserIn] |
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| | Bufton, Joshua C [VerfasserIn] |
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| | Borucu, Ufuk [VerfasserIn] |
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| | Shen, Dakang [VerfasserIn] |
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| | Becker, Jonas Philipp [VerfasserIn] |
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| | Lavysh, Daria [VerfasserIn] |
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| | Hentze, Matthias W. [VerfasserIn] |
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| | Kulozik, Andreas [VerfasserIn] |
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| | Neu-Yilik, Gabriele [VerfasserIn] |
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| | Schaffitzel, Christiane [VerfasserIn] |
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| Titel: | Blasticidin S inhibits mammalian translation and enhances production of protein encoded by nonsense mRNA |
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| Verf.angabe: | Kyle T Powers, Flint Stevenson-Jones, Sathish KN Yadav, Beate Amthor, Joshua C Bufton, Ufuk Borucu, Dakang Shen, Jonas P Becker, Daria Lavysh, Matthias W Hentze, Andreas E Kulozik, Gabriele Neu-Yilik and Christiane Schaffitzel |
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| E-Jahr: | 2021 |
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| Jahr: | 22 June 2021 |
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| Umfang: | 15 S. |
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| Fussnoten: | Gesehen am 06.10.2021 |
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| Titel Quelle: | Enthalten in: Nucleic acids research |
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| Ort Quelle: | Oxford : Oxford Univ. Press, 1974 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 49(2021), 13, Seite 7665-7679 |
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| ISSN Quelle: | 1362-4962 |
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| Abstract: | Deciphering translation is of paramount importance for the understanding of many diseases, and antibiotics played a pivotal role in this endeavour. Blasticidin S (BlaS) targets translation by binding to the peptidyl transferase center of the large ribosomal subunit. Using biochemical, structural and cellular approaches, we show here that BlaS inhibits both translation elongation and termination in Mammalia. Bound to mammalian terminating ribosomes, BlaS distorts the 3′CCA tail of the P-site tRNA to a larger extent than previously reported for bacterial ribosomes, thus delaying both, peptide bond formation and peptidyl-tRNA hydrolysis. While BlaS does not inhibit stop codon recognition by the eukaryotic release factor 1 (eRF1), it interferes with eRF1’s accommodation into the peptidyl transferase center and subsequent peptide release. In human cells, BlaS inhibits nonsense-mediated mRNA decay and, at subinhibitory concentrations, modulates translation dynamics at premature termination codons leading to enhanced protein production. |
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| DOI: | doi:10.1093/nar/gkab532 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1093/nar/gkab532 |
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| | DOI: https://doi.org/10.1093/nar/gkab532 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1772543691 |
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| Verknüpfungen: | → Zeitschrift |
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Blasticidin S inhibits mammalian translation and enhances production of protein encoded by nonsense mRNA / Powers, Kyle T. [VerfasserIn]; 22 June 2021 (Online-Ressource)
