A defined range of guard cell calcium oscillation parameters encodes stomatal movements

• Verfasst von: Allen, Gethyn J. [VerfasserIn]
• Verfasst von: Chu, Sarah P. [VerfasserIn]
• Verfasst von: Harrington, Carrie L. [VerfasserIn]
• Verfasst von: Schumacher, Karin [VerfasserIn]
• Verfasst von: Hoffmann, Thomas [VerfasserIn]
• Verfasst von: Tang, Yat Y. [VerfasserIn]
• Verfasst von: Grill, Erwin [VerfasserIn]
• Verfasst von: Schroeder, Julian I. [VerfasserIn]
• Titel: A defined range of guard cell calcium oscillation parameters encodes stomatal movements
• Verf.angabe: Gethyn J. Allen, Sarah P. Chu, Carrie L. Harrington, Karin Schumacher, Thomas Hoffmann, Yat Y. Tang, Erwin Grill & Julian I. Schroeder
• E-Jahr: 2001
• Jahr: 28 June 2001
• Umfang: 5 S.
• Teil: volume:411
• Teil: year:2001
• Teil: number:6841
• Teil: pages:1053-1057
• Teil: extent:5
• Fussnoten: Gesehen am 07.10.2021
• Titel Quelle: Enthalten in: Nature <London>
• Ort Quelle: London [u.a.] : Nature Publ. Group, 1869
• Jahr Quelle: 2001
• Band/Heft Quelle: 411(2001), 6841, Seite 1053-1057
• ISSN Quelle: 1476-4687
• DOI: doi:10.1038/35082575
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1772750999

Abstract

Oscillations in cytosolic calcium concentration ([Ca2+]cyt) are central regulators of signal transduction cascades1, although the roles of individual [Ca2+]cyt oscillation parameters in regulating downstream physiological responses remain largely unknown. In plants, guard cells integrate environmental and endogenous signals to regulate the aperture of stomatal pores2 and [Ca2+]cyt oscillations are a fundamental component of stomatal closure3,4. Here we systematically vary [Ca2+]cyt oscillation parameters in Arabidopsis guard cells using a ‘calcium clamp’3,5,6,7 and show that [Ca2+]cyt controls stomatal closure by two mechanisms. Short-term ‘calcium-reactive’ closure occurred rapidly when [Ca2+]cyt was elevated, whereas the degree of long-term steady-state closure was ‘calcium programmed’ by [Ca2+]cyt oscillations within a defined range of frequency, transient number, duration and amplitude. Furthermore, in guard cells of the gca2 mutant8, [Ca2+]cyt oscillations induced by abscisic acid and extracellular calcium had increased frequencies and reduced transient duration, and steady-state stomatal closure was abolished. Experimentally imposing [Ca2+]cyt oscillations with parameters that elicited closure in the wild type restored long-term closure in gca2 stomata. These data show that a defined window of guard cell [Ca2+]cyt oscillation parameters programs changes in steady-state stomatal aperture.