The Arabidopsis det3 mutant reveals a central role for the vacuolar H+-ATPase in plant growth and development

• Verfasst von: Schumacher, Karin [VerfasserIn]
• Verfasst von: Vafeados, Dionne [VerfasserIn]
• Verfasst von: McCarthy, Melissa [VerfasserIn]
• Verfasst von: Sze, Heven [VerfasserIn]
• Verfasst von: Wilkins, Thea [VerfasserIn]
• Verfasst von: Chory, Joanne [VerfasserIn]
• Titel: The Arabidopsis det3 mutant reveals a central role for the vacuolar H+-ATPase in plant growth and development
• Verf.angabe: Karin Schumacher, Dionne Vafeados, Melissa McCarthy, Heven Sze, Thea Wilkins, and Joanne Chory
• Jahr: 1999
• Umfang: 12 S.
• Teil: volume:13
• Teil: year:1999
• Teil: number:24
• Teil: pages:3259-3270
• Teil: extent:12
• Fussnoten: Im Titel ist "+" hochgestellt ; Gesehen am 07.10.2021
• Titel Quelle: Enthalten in: Genes & development
• Ort Quelle: Stanford, Calif. : HighWire Press, 1987
• Jahr Quelle: 1999
• Band/Heft Quelle: 13(1999), 24, Seite 3259-3270
• ISSN Quelle: 1549-5477
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Arabidopsis
• Sach-SW: brassinosteroids
• Sach-SW: cell expansion
• Sach-SW: det3
• Sach-SW: positional cloning
• Sach-SW: V-ATPase
• K10plus-PPN: 1772758957

Abstract

In all multicellular organisms growth and morphogenesis must be coordinated, but for higher plants, this is of particular importance because the timing of organogenesis is not fixed but occurs in response to environmental constraints. One particularly dramatic developmental juncture is the response of dicotyledonous seedlings to light. The det3 mutant of Arabidopsis develops morphologically as a light-grown plant even when it is grown in the dark. In addition, it shows organ-specific defects in cell elongation and has a reduced response to brassinosteroids (BRs). We have isolated the DET3 gene by positional cloning and provide functional and biochemical evidence that it encodes subunit C of the vacuolar H+-ATPase (V-ATPase). We show that the hypocotyl elongation defect in the det3 mutant is conditional and provide evidence that this is due to an alternative mechanism of V-ATPase assembly. Together with the expression pattern of the DET3 gene revealed by GFP fluorescence, our data provide in vivo evidence for a role for the V-ATPase in the control of cell elongation and in the regulation of meristem activity.